PLDA1/2 | Phospholipase D alpha 1/2

Name: PLDA1/2 | Phospholipase D alpha 1/2
SKU: AS12 2364
Price: 302 EUR
Availability: InStock

AS12 2364 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. oleracea, S. lycopersicum, L. luteus

PLDA1/2 | Phospholipase D alpha 1/2 in the group Antibodies Plant/Algal / Plant Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS12 2364)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PLD alpha 1, UniProt: Q38882, TAIR: AT3G15730 and PLD alpha 2, UniProt: Q9SSQ9, TAIR: AT1G52570

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 91,8 (PLD alpha 1 precursor), 91,5 (PLD alpha 2 precursor)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea

Predicted reactivity Brassica napus, Brassica rapa, Capsella rubella, Carica papaya, Gossypium hirsutum, Glycine max, Ricinus communis
Species of your interest not listed? Contact us

Not reactive in Olea europea, Zea mays

Application examples

Additional information

Background

Background PLDA1/2 (phospholipase D alpha 1/2) is an enzyme which hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond and plays an important tole in processes as phytochormone action and response to stress. This protein is highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds. Induced by ABA, ethylene, heavy metal, cold, salt and osmotic stresses.

Alternative names:PLDalpha1,PLD alpha 1,Choline phosphatase 1 PLDalpha, Phosphatidylcholine-hydrolyzing phospholipase D 1, Choline phosphatase 2, Phosphatidylcholine-hydrolyzing phospholipase D 2, PLDalpha2, PLD alpha 2.

Product citations

Selected references Kocourkova et al. (2020). Phospholipase Da1 mediates the high-Mg2+ stress response partially through regulation of K+ homeostasis. Plant Cell Environ. 2020 Jun 25.doi: 10.1111/pce.13831.

immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PLD alpha 1, UniProt: Q38882, TAIR: AT3G15730 and PLD alpha 2, UniProt: Q9SSQ9, TAIR: AT1G52570
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	91,8 (PLD alpha 1 precursor), 91,5 (PLD alpha 2 precursor)
Confirmed reactivity:	Arabidopsis thaliana, Brassica oleracea
predicted reactivity:	Brassica napus, Brassica rapa, Capsella rubella, Carica papaya, Gossypium hirsutum, Glycine max, Ricinus communis Species of your interest not listed? Contact us
not reactive in:	Olea europea, Zea mays
background:	PLDA1/2 (phospholipase D alpha 1/2) is an enzyme which hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond and plays an important tole in processes as phytochormone action and response to stress. This protein is highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds. Induced by ABA, ethylene, heavy metal, cold, salt and osmotic stresses. Alternative names:PLDalpha1,PLD alpha 1,Choline phosphatase 1 PLDalpha, Phosphatidylcholine-hydrolyzing phospholipase D 1, Choline phosphatase 2, Phosphatidylcholine-hydrolyzing phospholipase D 2, PLDalpha2, PLD alpha 2.
All references:	Kocourkova et al. (2020). Phospholipase Da1 mediates the high-Mg2+ stress response partially through regulation of K+ homeostasis. Plant Cell Environ. 2020 Jun 25.doi: 10.1111/pce.13831.