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PLDA1/2 | Phospholipase D alpha 1/2

AS12 2364 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. oleracea, S. lycopersicum, L. luteus

PLDA1/2 | Phospholipase D alpha 1/2 in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS12 2364)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PLD alpha 1, UniProt: Q38882,  TAIR: AT3G15730 and PLD alpha 2, UniProt: Q9SSQ9, TAIR: AT1G52570

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

91.8 (PLD alpha 1 precursor), 91.5 (PLD alpha 2 precursor)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea
Predicted reactivity Brassica napus, Brassica rapa, Capsella rubella, Carica papaya, Gossypium hirsutum, Glycine max, Ricinus communis
Species of your interest not listed? Contact us
Not reactive in Zea mays

Application examples

Additional information

Related products

Related products

AS09 556 | PLD | phospholipase D, rabbit antibody to native enzyme from Brassica oleracea

Plant protein extraction buffer

Secondary antibodies

Background

Background PLDA1/2 (phospholipase D alpha 1/2) is an enzyme which hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond and plays an important tole in processes as phytochormone action and response to stress. This protein is highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds. Induced by ABA, ethylene, heavy metal, cold, salt and osmotic stresses. 

Alternative names:PLDalpha1,PLD alpha 1,Choline phosphatase 1 PLDalpha, Phosphatidylcholine-hydrolyzing phospholipase D 1, Choline phosphatase 2,  Phosphatidylcholine-hydrolyzing phospholipase D 2, PLDalpha2, PLD alpha 2.

Product citations

Selected references Kocourkova et al. (2020). Phospholipase Da1 mediates the high-Mg2+ stress response partially through regulation of K+ homeostasis. Plant Cell Environ. 2020 Jun 25.doi: 10.1111/pce.13831.

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