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PRO4,5 | Profilin-4,5 (clone mAbPRF45a (2-B8))

AS16 3143  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana

PRO4,5 | Profilin-4,5 (clone mAbPRF45a (2-B8)) in the group Antibodies for Plant/Algal  / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS16 3143)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen Profilin 4 (PRF4) from Arabidopsis thaliana, UniProt: Q38905, TAIR: AT4G29340

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Total IgG
Format Liquid in 0.1M PBS, pH 7.4, with 0.15M Sodium Chloride, and 0.05% Sodium Azide
Quantity 100 µg
Storage Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC), Western blot (WB)
Expected | apparent MW

14 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-profilin 4 antibodies

5 µg of recombinant profilins from Arabidopsis thaliana and 20 µg of total protein from whole plant extract. Coomassie blue stained gel (bottom) shows equal loading of recombinant profilins.

Additional information

Additional information

Exact working dilution needs to be determined by end user.

This antibody reacts with both, PRF4 (profilin 4) and PRF5 (profilin 5).

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Background

Background Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin also interacts with membrane polyphospho-inositides, the Arp 2/3 complex, short proline rich stretches, and other ligands.

Product citations

Selected references Kandasamy, M.K., et al. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52(1):22-32.
McKinney, E.C., et al. (2001) Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13(5): 1179-91.

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