Name:
Phone:
E-mail:
Address:






PSA2 | Photosystem I assembly factor 2

AS13 2654 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PSA2 | Photosystem I assembly factor 2 in the group Antibodies Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS13 2654)
PSA2 | Photosystem I assembly factor 2



DATA SHEET IN PDF

Qty: 
302
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen recombinant protein corresponding to amino acids 87 to 186 of Arabidopsis thaliana PSA2, UniProt:O64750, TAIR: AT2G34860
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

20 kDa | 10 kDa (processed form)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application example

western blot using anti-PSA2 antibodies

Respective amounts of a leaf total cell extract from Arabidopsis thaliana loaded on total chlorophyll µg/µl were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesty of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands

Additional information

Additional information PSA2 | Photosystem I assembly factor 2

Related products

Background

Background PSA2 (Photosystem I assembly factor 2) is a protein coded by a gene belonging to the "Green Cut" set, found only in green algae and plants but not in non-photosynthetic organisms. PSA2 is localized in thylakoid lumen.

Product citations

Selected references Fristedt et al. (2014). A Thylakoid Membrane Protein Harboring a DnaJ-type Zinc Finger Domain is Required for Photosystem I Accumulation in Plants. J Biol Chem. 2014 Sep 16. pii: jbc.M114.587758.
immunogen: recombinant protein corresponding to amino acids 87 to 186 of Arabidopsis thaliana PSA2, UniProt:O64750, TAIR: AT2G34860
Reconstitution: For reconstitution add 50 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 50 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Immunoprecipitation (IP), Western blot (WB)
recommended dilution: 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]:

20 kDa | 10 kDa (processed form)

Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Brassica rapa
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer):

application example

western blot using anti-PSA2 antibodies

Respective amounts of a leaf total cell extract from Arabidopsis thaliana loaded on total chlorophyll µg/µl were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesty of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands

additional information: PSA2 | Photosystem I assembly factor 2
background: PSA2 (Photosystem I assembly factor 2) is a protein coded by a gene belonging to the "Green Cut" set, found only in green algae and plants but not in non-photosynthetic organisms. PSA2 is localized in thylakoid lumen.
All references: Fristedt et al. (2014). A Thylakoid Membrane Protein Harboring a DnaJ-type Zinc Finger Domain is Required for Photosystem I Accumulation in Plants. J Biol Chem. 2014 Sep 16. pii: jbc.M114.587758.

Related products: PSA2 | Photosystem I assembly factor 2

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS13 2641 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS13 2683  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabi...
211 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €