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CGL40

AS13 2641 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CGL40 in the group Antibodies for Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS13 2641)

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

full length, recombinant CGL40, UniProt: Q8LFL5 , TAIR:  At1g49975

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

14.73 kDa-cTP: 8.42 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Capsella rubella, Brassica pekinensis
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-CGL40 antibodies

3 μg of total protein from Arabidopsis thaliana extracted with were separated on 15 % SDS-PAGE  and blotted 1h to PVDF. Blots were blocked with 10% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 15 min and 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG, HRP-conjugated) diluted to 1:15 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Antibody incubation can be also conducted for 1 h/RT.

Courtesy of Dr. Rikard Fristedt, UCLA, USA

Additional information

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Background

Background

CGL40 is a very conserved protein involved in photosynthesis and its function is far not identified.

Product citations

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