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CGL40

AS13 2641 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CGL40 in the group Antibodies Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS13 2641)
CGL40



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Product Information

Immunogen

full length, recombinant CGL40, UniProt: Q8LFL5 , TAIR:  At1g49975

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 14,73 kDa-cTP: 8,42 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Capsella rubella, Brassica pekinensis
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application example

western blot using anti-CGL40 antibodies

3 μg of total protein from Arabidopsis thaliana extracted with were separated on 15 % SDS-PAGE  and blotted 1h to PVDF. Blots were blocked with 10% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 15 min and 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG, HRP-conjugated) diluted to 1:15 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Antibody incubation can be also conducted for 1 h/RT.

Courtesy of Dr. Rikard Fristedt, UCLA, USA

Additional information

Related products

Background

Background

CGL40 is a very conserved protein involved in photosynthesis and its function is far not identified.

Product citations

calculated | apparent molecular mass [kDa]: 14,73 kDa-cTP: 8,42 kDa
Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

full length, recombinant CGL40, UniProt: Q8LFL5 , TAIR:  At1g49975

Purity: Serum
Quantity: 50 ĩl
recommended dilution: 1 : 1000 (WB)
Reconstitution: For reconstitution add 50 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Capsella rubella, Brassica pekinensis
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer):

application example

western blot using anti-CGL40 antibodies

3 μg of total protein from Arabidopsis thaliana extracted with were separated on 15 % SDS-PAGE  and blotted 1h to PVDF. Blots were blocked with 10% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 15 min and 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG, HRP-conjugated) diluted to 1:15 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Antibody incubation can be also conducted for 1 h/RT.

Courtesy of Dr. Rikard Fristedt, UCLA, USA

background:

CGL40 is a very conserved protein involved in photosynthesis and its function is far not identified.

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