CGL160 | Conserved in green lineage 160

part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279, TAIR: At2g31040

38.6 | 34 kDa (without transit peptide)

application example

Respective amounts of µg/ul of chlorophyll from Arabidopsis thaliana leaf extracted with sample buffer (2% SDS, 8% sucrose, 0.2mM EDTA, 10mM Tris HCl (pH 6.8) 4% beta-mercaptoethanol) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.

Courtesy of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands

AS08 370 | Anti-ATPase | ATP synthase, whole enzyme, rabbit antibody

collection of antibodies to GreenCut proteins

Plant and algal protein extraction buffer

Secondary antibodies

CGL160 (Conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast. It interacts with components of the CF₁ sub-complex. Synonymes: ATCGL160.