Name:
Phone:
E-mail:
Message


 


CGL160 | Conserved in green lineage 160

AS12 1853 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CGL160 |  Conserved in green lineage 160 in the group Antibodies for Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS12 1853)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279, TAIR: At2g31040

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

38.6 | 34 kDa (without transit peptide)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-CGL160 antibodies
Respective amounts of
µg/ul of chlorophyll from Arabidopsis thaliana leaf extracted with sample buffer (2% SDS, 8% sucrose, 0.2mM EDTA, 10mM Tris HCl (pH 6.8) 4% beta-mercaptoethanol) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.

Courtesy of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands

Additional information

Related products

Related products

AS08 370 | Anti-ATPase | ATP synthase, whole enzyme, rabbit antibody

collection of antibodies to GreenCut proteins

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

CGL160 (Conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast. It interacts with components of the CF1 sub-complex. Synonymes: ATCGL160.

Product citations

Selected references Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.
Fristedt et al. (2015). The Thylakoid Membrane Protein CGL160 Supports CF1CF0 ATP Synthase Accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.

Related products: CGL160 | Conserved in green lineage 160

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS08 370  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: ...
373 €
AS13 2683  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabi...
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €