CGL78 | YCF54
AS10 936 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, Synechocystis sp. PCC6803
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Customer reviews |
Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
200 ĩl
Reconstitution
For reconstitution add 200 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
24 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana , Chlamydomonas reinhardtii, Synechocystis sp. PCC 6803
Predicted reactivity
Species of your interest not listed? Contact us
Not reactive in
Hordeum vulgare
Application examples
Application examples
Application information
15 µg of total protein from Arabidopsis thaliana (ecotype Col-0), Chlamydomonas reinhardtii (strain 4A+) and Synechocystis sp. (strain PCC6803 / Kazusa), extracted with 56 mM Na2CO3, 56 mM DTT, 1 % (w/v) SDS, 12 % (w/v) Sucrose, 2 mM EDTA) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose membrane. Blot was blocked with 2% milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 1% milk powder in TBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturers instructions. Exposure time was 3 minutes.
Courtesy of Dr. Annabel Salinas Hartwig, Humboldt University, Germany
15 µg of total protein from Arabidopsis thaliana (ecotype Col-0), Chlamydomonas reinhardtii (strain 4A+) and Synechocystis sp. (strain PCC6803 / Kazusa), extracted with 56 mM Na2CO3, 56 mM DTT, 1 % (w/v) SDS, 12 % (w/v) Sucrose, 2 mM EDTA) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose membrane. Blot was blocked with 2% milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 1% milk powder in TBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturers instructions. Exposure time was 3 minutes.
Courtesy of Dr. Annabel Salinas Hartwig, Humboldt University, Germany
Additional information
Additional information
Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.
Please, omitt SDS from transfer buffer and reduce transfer time to 45 min, Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane
Background
Background
CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.
Product citations
Selected references
Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
Related products: CGL78 | YCF54
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD...
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
10 % until...
10 % until...
206 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | 
