Anti-CGL78 | YCF54

Product no: AS10 936

AS10 936  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. reinhardtii, Synechocystis sp. PCC6803

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    recombinant fragment of Arabidopsis thaliana CGL78 UniProt: Q9LVM3  TAIR: At5g58250

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    24 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana , Chlamydomonas reinhardtii, Synechocystis sp. PCC 6803
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in:

    Hordeum vulgare

  • Application Examples
  • Application information

    western blot detection of CGL78 protein
    15 µg of total protein from Arabidopsis thaliana (ecotype Col-0), Chlamydomonas reinhardtii (strain 4A+) and Synechocystis sp. (strain PCC6803 / Kazusa), extracted with 56 mM Na2CO3, 56 mM DTT, 1 % (w/v) SDS, 12 % (w/v) Sucrose, 2 mM EDTA) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose membrane. Blot was blocked with 2% milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 1% milk powder in TBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturers instructions. Exposure time was 3 minutes.

    Courtesy of Dr. Annabel Salinas Hartwig, Humboldt University, Germany
  • Additional Information
  • Additional information:

    Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.

    Additional information (application): Please, omitt SDS from transfer buffer and reduce transfer time to 45 min, Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane
  • Background
  • Background: CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.
  • Product Citations
  • Selected references: Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection

  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (1)
    Annabel Salinas | 2013-04-17
    0 | 0
    The antibody detects specifically YCF54 in Arabidopsis (apparent MW ca. 20 kDa).


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