Name:
Phone:
E-mail:
Message


 


CGL78 | YCF54

AS10 936  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. reinhardtii, Synechocystis sp. PCC6803

CGL78 | YCF54 in the group Antibodies for Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS10 936)

DATA SHEET IN PDF

Qty: 
373
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

recombinant fragment of Arabidopsis thaliana CGL78 UniProt: Q9LVM3  TAIR: At5g58250

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

24 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana , Chlamydomonas reinhardtii, Synechocystis sp. PCC 6803
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in

Hordeum vulgare

Application examples

Application examples Application information

western blot detection of CGL78 protein
15 µg of total protein from Arabidopsis thaliana (ecotype Col-0), Chlamydomonas reinhardtii (strain 4A+) and Synechocystis sp. (strain PCC6803 / Kazusa), extracted with 56 mM Na2CO3, 56 mM DTT, 1 % (w/v) SDS, 12 % (w/v) Sucrose, 2 mM EDTA) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose membrane. Blot was blocked with 2% milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 1% milk powder in TBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturers instructions. Exposure time was 3 minutes.

Courtesy of Dr. Annabel Salinas Hartwig, Humboldt University, Germany

Additional information

Additional information

Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.

Please, omitt SDS from transfer buffer and reduce transfer time to 45 min. Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane.

Related products

Related products

AS06 122 | CRD1 | cyanobacterial homolog of plant CHL27 cyclase

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.

Product citations

Selected references Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.

Related products: CGL78 | YCF54

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection...
From 15 €
AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD...
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
...
195 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
...
190 €