CRD1 | Cyanobacterial homolog of plant CHL27 cyclase
AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD1) and plants (CHL27) including A.thaliana, H.vulgare, N. tabacum, P.sativum, P. patens, Synechocystis sp. PCC6803
compartment marker of chloroplast thylakoid and envelope membranes
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47 | 40 kDa (Arabidopsis thaliana)
Cyanobacteria, Gossyium hirsutum, Euphorbia esula, Hordeum vulgare, Nannochloropsis gaditana, Ricinus communis
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Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 32198392
Journal: Nat Commun
Figure Number: 2H,J
Published Date: 2020-03-20
First Author: Wang, P., Richter, A. S., et al.
Impact Factor: 13.783Open Publication
BCM1 is a positive regulator of Chl biosynthesis in Arabidopsis.a Representative images of 28-day-old WT, bcm1 and BCM1-OX seedlings grown under short-day normal light (120??mol photon?m?2?s?1) conditions. Scale bar, 1?cm. b Scheme of TBS in higher plants. c–g Relative ALA synthesis rate (c), Proto (d), Mg-porphyrin (e), Chl (f), and heme (g) levels in 18-day-old WT, bcm1, and BCM1-OX seedlings grown under the same conditions as in a. Error bars represent SD of eight, three, four, twelve, and six biological replicates, respectively. Letters above histograms indicate significant differences as determined by Tukey’s HSD method (P?0.05). h Steady-state levels of the indicated proteins in seedlings analyzed in a were determined by immunoblot analysis using the indicated antibodies. i Steady-state levels of the indicated proteins in 28-day-old WT, gun5-1, cch, gun4-1, gun4-3, chl27, and ch1-2 seedlings, grown under short-day low light (70??mol photons?m?2?s?1) conditions were quantified by immunoblotting using the indicated antibodies. A representative image of the Arabidopsis seedlings analyzed in i is shown. In h, i, Ponceau S-stained membrane strips bearing RbcL were used as loading controls. Numbers below immunoblots represent normalized protein abundances relative to WT seedlings.
[compartment marker] of chloroplast thylakoid and envelope membranes
Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria
Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.
Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.
Canniffe et al. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. Biochem J. 2014 Jun 19.
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