CRD1 | Cyanobacterial homolog of plant CHL27 cyclase

AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD1) and plants (CHL27) including A.thaliana, H.vulgare, N. tabacum, P.sativum, P. patens, Synechocystis sp. PCC6803 | cellular [compartment marker] of chloroplast thylakoid and envelope membranes

Product Information

Immunogen

residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA UniProt: Q9M591,TAIR: At3g56940

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW

47 | 40 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidosps thaliana, Horderum vulgare, Nicotiana tabacum, Pisum sativum, Physcomitrella patens, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27), Synechocystis PCC 6803, anoxygenic phototrophs (proeobacteria): Congregibacter litoralis, Roseobacter litoralis, green non-sulfur bacterium: Chloroflexus aurantiacus, photosynthetic bacterium: Rubriviavax gelatinosus

Predicted reactivity

Cyanobacteria, Gossyium hirsutum, Euphorbia esula, Hordeum vulgare, Ricinus communis

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

[compartment marker] of chloroplast thylakoid and envelope membranes

Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria

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Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant protein extraction buffer

Background

Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.

Product citations

Selected references Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.
Canniffe et al. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. Biochem J. 2014 Jun 19.

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