Anti-CRD1 | Cyanobacterial homolog of plant CHL27 cyclase

Product no: AS06 122

AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD1) and plants (CHL27) including A.thaliana, H.vulgare, N. tabacum, P.sativum, P. patens, Synechocystis sp. PCC6803

compartment marker of chloroplast thylakoid and envelope membranes

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  • Product Info
  • Immunogen:

    residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA UniProt: Q9M591,TAIR: At3g56940

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW:

    47 | 40 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidosps thaliana, Horderum vulgare, Nicotiana tabacum, Pisum sativum, Physcomitrium patens, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27), Synechocystis PCC 6803, anoxygenic phototrophs (proeobacteria): Congregibacter litoralis, Roseobacter litoralis, green non-sulfur bacterium: Chloroflexus aurantiacus, photosynthetic bacterium: Rubriviavax gelatinosus
    Predicted reactivity:

    Cyanobacteria, Gossyium hirsutum, Euphorbia esula, Hordeum vulgare, Nannochloropsis gaditana, Ricinus communis

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application examples:

    Reactant: Arabidopsis thaliana (Thale cress)

    Application: Western Blotting

    Pudmed ID: 32198392

    Journal: Nat Commun

    Figure Number: 2H,J

    Published Date: 2020-03-20

    First Author: Wang, P., Richter, A. S., et al.

    Impact Factor: 13.783

    Open Publication

    BCM1 is a positive regulator of Chl biosynthesis in Arabidopsis.a Representative images of 28-day-old WT, bcm1 and BCM1-OX seedlings grown under short-day normal light (120??mol photon?m?2?s?1) conditions. Scale bar, 1?cm. b Scheme of TBS in higher plants. c–g Relative ALA synthesis rate (c), Proto (d), Mg-porphyrin (e), Chl (f), and heme (g) levels in 18-day-old WT, bcm1, and BCM1-OX seedlings grown under the same conditions as in a. Error bars represent SD of eight, three, four, twelve, and six biological replicates, respectively. Letters above histograms indicate significant differences as determined by Tukey’s HSD method (P?<?0.05). h Steady-state levels of the indicated proteins in seedlings analyzed in a were determined by immunoblot analysis using the indicated antibodies. i Steady-state levels of the indicated proteins in 28-day-old WT, gun5-1, cch, gun4-1, gun4-3, chl27, and ch1-2 seedlings, grown under short-day low light (70??mol photons?m?2?s?1) conditions were quantified by immunoblotting using the indicated antibodies. A representative image of the Arabidopsis seedlings analyzed in i is shown. In h, i, Ponceau S-stained membrane strips bearing RbcL were used as loading controls. Numbers below immunoblots represent normalized protein abundances relative to WT seedlings.

  • Additional Information
  • Additional information:

    [compartment marker] of chloroplast thylakoid and envelope membranes

    Additional information (application):

    Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria

  • Background
  • Background:

    Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.

  • Product Citations
  • Selected references: Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254.
    Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.
    Canniffe et al. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. Biochem J. 2014 Jun 19.
    Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (2)
    Annabel Salinas | 2013-01-23
    0 | 0
    The antibody detects specifically CHL27 in Arabidopsis (apparent MW ca. 40 kDa) and tobacco (apparent MW ca. 45 kDa)
    Thmas Wallner | 2009-05-20
    0 | 0
    The antibody detects the two Synechocystis homologues of the plant oxidative cyclase: sll1214/ ChlAI and sll1874/ ChlAII


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