CRD1 | Cyanobacterial homolog of plant CHL27 cyclase
AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD1) and plants (CHL27) including A.thaliana, H.vulgare, N. tabacum, P.sativum, P. patens, Synechocystis sp. PCC6803
compartment marker of chloroplast thylakoid and envelope membranes
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Product Information
residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA UniProt: Q9M591,TAIR: At3g56940
47 | 40 kDa (Arabidopsis thaliana)
Reactivity
Cyanobacteria, Gossyium hirsutum, Euphorbia esula, Hordeum vulgare, Nannochloropsis gaditana, Ricinus communis
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Application examples
Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 32198392
Journal: Nat Commun
Figure Number: 2H,J
Published Date: 2020-03-20
First Author: Wang, P., Richter, A. S., et al.
Impact Factor: 13.783
Open PublicationBCM1 is a positive regulator of Chl biosynthesis in Arabidopsis.a Representative images of 28-day-old WT, bcm1 and BCM1-OX seedlings grown under short-day normal light (120??mol photon?m?2?s?1) conditions. Scale bar, 1?cm. b Scheme of TBS in higher plants. c–g Relative ALA synthesis rate (c), Proto (d), Mg-porphyrin (e), Chl (f), and heme (g) levels in 18-day-old WT, bcm1, and BCM1-OX seedlings grown under the same conditions as in a. Error bars represent SD of eight, three, four, twelve, and six biological replicates, respectively. Letters above histograms indicate significant differences as determined by Tukey’s HSD method (P?
Additional information
[compartment marker] of chloroplast thylakoid and envelope membranes
Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria
Background
Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.
Product citations
Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.
Canniffe et al. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. Biochem J. 2014 Jun 19.
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