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CRD1 | Cyanobacterial homolog of plant CHL27 cyclase

AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD1) and plants (CHL27) including A.thaliana, H.vulgare, N. tabacum, P.sativum, P. patens, Synechocystis sp. PCC6803

compartment marker of chloroplast thylakoid and envelope membranes

CRD1 | Cyanobacterial homolog of plant CHL27 cyclase in the group Antibodies Plant/Algal  / Photosynthesis  / Chlorophyll at Agrisera AB (Antibodies for research) (AS06 122)
CRD1 | Cyanobacterial homolog of plant CHL27 cyclase



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Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA UniProt: Q9M591,TAIR: At3g56940

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

47 | 40 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidosps thaliana, Horderum vulgare, Nicotiana tabacum, Pisum sativum, Physcomitrium patens, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27), Synechocystis PCC 6803, anoxygenic phototrophs (proeobacteria): Congregibacter litoralis, Roseobacter litoralis, green non-sulfur bacterium: Chloroflexus aurantiacus, photosynthetic bacterium: Rubriviavax gelatinosus
Predicted reactivity

Cyanobacteria, Gossyium hirsutum, Euphorbia esula,  Hordeum vulgare, Nannochloropsis gaditana, Ricinus communis


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 32198392

Journal: Nat Commun

Figure Number: 2H,J

Published Date: 2020-03-20

First Author: Wang, P., Richter, A. S., et al.

Impact Factor: 13.783

Open Publication

BCM1 is a positive regulator of Chl biosynthesis in Arabidopsis.a Representative images of 28-day-old WT, bcm1 and BCM1-OX seedlings grown under short-day normal light (120??mol photon?m?2?s?1) conditions. Scale bar, 1?cm. b Scheme of TBS in higher plants. c–g Relative ALA synthesis rate (c), Proto (d), Mg-porphyrin (e), Chl (f), and heme (g) levels in 18-day-old WT, bcm1, and BCM1-OX seedlings grown under the same conditions as in a. Error bars represent SD of eight, three, four, twelve, and six biological replicates, respectively. Letters above histograms indicate significant differences as determined by Tukey’s HSD method (P?

Additional information

Additional information

[compartment marker] of chloroplast thylakoid and envelope membranes

Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria

Related products

Related products

AS10 936 | Anti-CGL78 | YCF54, rabbit antibodies

AS05 084 | Anti-PsbA rabbit antibodies, marker of thylakoid membrane

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant protein extraction buffer

Background

Background

Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.

Product citations

Selected references Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254.
Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.
Canniffe et al. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. Biochem J. 2014 Jun 19.

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