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Rabbit anti-Bovine IgG (H&L), HRP conjugated

AS16 3253 | Clonality: Polyclonal | Host: Rabbit| Reactivity: Bovine IgG (H&L)
Rabbit anti-Bovine IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Bovine / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS16 3253)

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215 €
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Product Information

Immunogen Purified bovine IgG, whole molecule
Host Rabbit
Clonality Polyclonal
Purity Affinity purified using solid phase bovine IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage Store freeze-dried powder at 2-8 °C.  For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Tested applications ELISA (ELISA), Immunohistochemistry(IHC), Western blot (WB)
Recommended dilution 1 : 200-1 : 5 000 (ELISA), 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5 000 (WB)

Reactivity

Confirmed reactivity Heavy chains on bovine IgG and light chains on all bovine immunoglobulins.
Not reactive in Non-immunoglobulin bovine serum proteins.

Application examples

Additional information

Additional information

Antibody purity is > 95% based on SDS-PAGE.

Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) ProClin 150 is added as a preservative.

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Background

Background Rabbit anti-Bovine IgG (H&L), HRP conjugated, is a secondary antibody conjugated to HRP which binds to Bovine IgG (H&L) in immunological assays.

Product citations

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