Rabbit anti-Bovine IgG (H&L), HRP conjugated
AS16 3253 | Clonality: Polyclonal | Host: Rabbit| Reactivity: Bovine IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
10 % until end of 2023. Use discount code: Conj10

Data sheet | Product citations | Add review |
Product Information
Immunogen
Purified bovine IgG, whole molecule
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified rabbit IgG.
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Storage
Store freeze-dried powder at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Tested applications
ELISA (ELISA), Immunohistochemistry(IHC), Western blot (WB)
Recommended dilution
1 : 200-1 : 5 000 (ELISA), 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5 000 (WB)
Reactivity
Confirmed reactivity
Heavy chains on bovine IgG and light chains on all bovine immunoglobulins
Not reactive in
Non-immunoglobulin bovine serum proteins
Application examples
Additional information
Additional information
Antibody purity is > 95% based on SDS-PAGE.
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) ProClin 150 is added as a preservative.
Background
Background
Rabbit anti-Bovine IgG (H&L), HRP conjugated, is a secondary antibody conjugated to HRP which binds to Bovine IgG (H&L) in immunological assays.
Product citations
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