Rabbit anti-Bovine IgG (H&L), HRP conjugated

AS16 3253 | Clonality: Polyclonal | Host: Rabbit| Reactivity: Bovine IgG (H&L)


product information
Background		Rabbit anti-Bovine IgG (H&L), HRP conjugated, is a secondary antibody conjugated to HRP which binds to Bovine IgG (H&L) in immunological assays.
Immunogen		Purified Bovine IgG, whole molecule
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store freeze-dried powder at 2-8 °C.
Tested applications		ELISA (ELISA), Immunohistochemistry(IHC), Western blot (WB)
Related products		other anti-bovine, HRP-conjugated, secondary antibodies
Additional information		Antibody purity is > 95% based on SDS-PAGE. Affinity purified using solid phase Bovine IgG (H&L). Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative. Based on IEP, this antibody reacts with: • heavy (γ) chains on bovine IgG • light chains on all bovine immunoglobulins . Based on IEP, no reactivity is observed to: • non-immunoglobulin bovine serum immunoglobulins .

application information
Recommended dilution		1 : 200-1 : 5 000 (ELISA), 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5 000 (WB). The optimal working dilution should be determined by the investigator.
Expected \| apparent MW
Confirmed reactivity		Bovine IgG (H&L)
Predicted reactivity
Not reactive in
Additional information
Selected references		To be added when available, antibody released in April 2016.

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