Rabbit anti-Chicken IgY (H&L), TRITC conjugated

Product no: AS10 1490
AS10 1490 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken IgG/Y (H&L)
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  • Product Info
  • Immunogen: Purified Chicken IgG/Y (H&L)
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified rabbit IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
  • Additional Information
  • Additional information:

    Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

    Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins

  • Background
  • Background: Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to TRITC , which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y. Tetramethylrhodamine-5-isothiocyanate (TRITC) has  Amax = 550 nm; Emax = 570 nm.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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