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Rabbit anti-Human IgG (H&L), FITC conjugated

AS10 1502 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human IgG (H&L)
Rabbit anti-Human IgG (H&L), FITC conjugated in the group Secondary Antibodies / Anti-Human / Fluorescent / IgG at Agrisera AB (Antibodies for research) (AS10 1502)

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Product Information

Immunogen Purified human IgG (H&L) CAA10835
Host Rabbit
Clonality Polyclonal
Purity Affinity purified rabbit IgG
Format Liquid
Quantity 2 mg
Storage Store at 2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Recommended dilution The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.

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Background

Background Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to FITC, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm

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