Rabbit anti-Human IgM (µ chain), TRITC conjugated
- Product Info
Immunogen: Purified human IgM (m chain) AAC37537 Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified rabbit IgG. Format: Liquid Quantity: 1 mg Storage: Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard. Recommended dilution: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
- Additional Information
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Background: Rabbit anti-human IgM (µ chain)is a secondary antibody conjugated to TRITC, which binds to human IgM in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
- Antibody protocols
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