Rabbit anti-Llama IgG (H&L), ALP conjugated
AS10 1514 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Llama IgG (H&L)
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Product Information
Immunogen
Purified llama IgG (H&L) AAQ19986
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified rabbit IgG
Format
Liquid
Quantity
0.5 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Reactivity
Application examples
Additional information
Additional information
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis no reactivity is observed to: non-immunoglobulin llama serum proteins.
This antibody will react with VHH of llama IgG's.
Background
Background
Rabbit anti-llama IgG (H&L) is a secondary antibody conjugated to ALP, which binds to in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product citations
Selected references
Alharbi et al. (2019). Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019 Nov 8;9(1):16292. doi: 10.1038/s41598-019-52730-4.
Aharbi et al. (2019). Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019 Nov 8;9(1):16292. doi: 10.1038/s41598-019-52730-4.
Aharbi et al. (2019). Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019 Nov 8;9(1):16292. doi: 10.1038/s41598-019-52730-4.
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