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Rabbit anti-Rat IgG (H&L), ALP conjugated

AS10 1521 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rat IgG (H&L)
Rabbit anti-Rat IgG (H&L), ALP conjugated in the group Secondary Antibodies / Anti-Rat / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1521)

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195
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Product Information

Immunogen Purified rat IgG (H&L)
Host Rabbit
Clonality Polyclonal
Purity Affinity purified rabbit IgG
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)

Reactivity

Application examples

Additional information

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.

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Background

Background Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).

Product citations

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