RecA | Recombinase A

AS21 4542 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

Product Information

Immunogen Hihgly purified, full length (352 amino acids) RecA protein of E.coli, UniProt: P0A7G6

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS. Contains 50% glycerol, filter sterilized.

Format Liquid

Quantity 50 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Indirect immunofluorescent (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1: 600 (IP), 1: 1000 - 1: 3000 (WB)

Expected | apparent MW 38 | 37 kDa

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using RecA antibodies

Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4). Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.

Additional information

Additional information RecA | Recombinase A

Background

Background RecA (Recombinase A) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.

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Picture (footer):	Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4). Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.
calculated \| apparent molecular mass [kDa]:	38 \| 37 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Hihgly purified, full length (352 amino acids) RecA protein of E.coli, UniProt: P0A7G6
Purity:	Immunogen affinity purified serum in PBS. Contains 50% glycerol, filter sterilized.
Quantity:	50 µg
recommended dilution:	1: 600 (IP), 1: 1000 - 1: 3000 (WB)
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Indirect immunofluorescent (IF), Immunoprecipitation (IP), Western blot (WB)
background:	RecA (Recombinase A) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.
additional information:	RecA \| Recombinase A
Confirmed reactivity:	Escherichia coli