IKP

RecA | Recombinase A

Product no: AS21 4542
AS21 4542 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
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  • Product Info
  • Immunogen: Hihgly purified, full length (352 amino acids) RecA protein of E.coli, UniProt: P0A7G6
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS. Contains 50% glycerol, filter sterilized.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Indirect immunofluorescent (IF), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1: 600 (IP), 1: 1000 - 1: 3000 (WB)
    Expected | apparent MW: 38 | 37  kDa
  • Reactivity
  • Confirmed reactivity: Escherichia coli
  • Application Examples
  • Western blot using RecA antibodies

    Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4).  Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.
  • Additional Information
  • Additional information: RecA | Recombinase A
  • Background
  • Background: RecA (Recombinase A) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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