RecA | Recombinase A
AS21 4542 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

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Product Information
Immunogen
Hihgly purified, full length (352 amino acids) RecA protein of E.coli, UniProt: P0A7G6
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS. Contains 50% glycerol, filter sterilized.
Format
Liquid
Quantity
50 ĩg
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Indirect immunofluorescent (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1: 600 (IP), 1: 1000 - 1: 3000 (WB)
Expected | apparent MW
38 | 37 kDa
Reactivity
Confirmed reactivity
Escherichia coli
Application examples
Application examples

Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4). Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.

Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4). Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.
Additional information
Additional information
RecA | Recombinase A
Background
Background
RecA (Recombinase A) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.
Product citations
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