LexA | LexA repressor

Name: LexA | LexA repressor
Brand: IKP
SKU: AS21 4541
Price: 342 EUR
Availability: InStock

AS21 4541 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

LexA | LexA repressor in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4541)

Product Information

Immunogen Full-size highly-purified recombinant LexA protein, UniProt: P0A7C2

Host Rabbit

Clonality Polyclonal

Purity Serum. Contains 0.05% sodium azide.

Format Liquid

Quantity 50 µl

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)

Recommended dilution 1: 1000 - 1: 3000 (WB)

Expected | apparent MW 22,3 | 23 kDa

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-LexA (E.coli) antibodies

Escherichia coli cells (1 × 10 8 cells/mL) were lysed in lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% SDS, and bacterial protease inhibitor cocktail (Sigma) and boiled followed by gel electrophoresis on 15% SDS-PAGE gels. Anti-LexA antibodies were used at 1: 1000.

For Western blot anti-LexA antibody can be combined with LexA protein as a positive control.

Additional information

Additional information This product can be used in:

studies of SOS regulation in E.coli
detection of bait constructs in yeast two-hybrid system
immunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)
immunoprecipiation and ChIP

Background

Background LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.

Alternative names: exrA, spr, tsl, umuA

Product citations

Selected references Hishida et al (2004) Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004 Aug 1;18(15):1886-97. doi: 10.1101/gad.1223804. PMID: 15289460; PMCID: PMC517408.

immunogen:	Full-size highly-purified recombinant LexA protein, UniProt: P0A7C2
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum. Contains 0.05% sodium azide.
Format:	Liquid
Quantity:	50 µl
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)
recommended dilution:	1: 1000 - 1: 3000 (WB)
calculated \| apparent molecular mass [kDa]:	22,3 \| 23 kDa
Confirmed reactivity:	Escherichia coli
Picture (footer):	Escherichia coli cells (1 × 10 8 cells/mL) were lysed in lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% SDS, and bacterial protease inhibitor cocktail (Sigma) and boiled followed by gel electrophoresis on 15% SDS-PAGE gels. Anti-LexA antibodies were used at 1: 1000. For Western blot anti-LexA antibody can be combined with LexA protein as a positive control.
additional information:	This product can be used in: studies of SOS regulation in E.coli detection of bait constructs in yeast two-hybrid system immunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde) immunoprecipiation and ChIP
background:	LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo. Alternative names: exrA, spr, tsl, umuA
All references:	Hishida et al (2004) Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004 Aug 1;18(15):1886-97. doi: 10.1101/gad.1223804. PMID: 15289460; PMCID: PMC517408.