LexA | LexA repressor
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Escherichia coli cells (1 × 10 8 cells/mL) were lysed in lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% SDS, and bacterial protease inhibitor cocktail (Sigma) and boiled followed by gel electrophoresis on 15% SDS-PAGE gels. Anti-LexA antibodies were used at 1: 1000.
For Western blot anti-LexA antibody can be combined with LexA protein as a positive control.
- studies of SOS regulation in E.coli
detection of bait constructs in yeast two-hybrid system
- immunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)
- immunoprecipiation and ChIP
Alternative names: exrA, spr, tsl, umuA
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