LexA | LexA repressor

AS21 4541 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
LexA | LexA repressor in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4541)


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Full-size highly-purified recombinant LexA protein, UniProt: P0A7C2
Host Rabbit
Clonality Polyclonal
Purity Serum. Contains 0.05% sodium azide.
Format Liquid
Quantity 50 ĩl
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)
Recommended dilution 1: 1000 - 1: 3000 (WB)
Expected | apparent MW 22,3 | 23 kDa


Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-LexA (E.coli) antibodies

Escherichia coli cells (1 × 10 8 cells/mL) were lysed in lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% SDS, and bacterial protease inhibitor cocktail (Sigma) and boiled followed by gel electrophoresis on 15% SDS-PAGE gels. Anti-LexA antibodies were used at 1: 1000. 

For Western blot anti-LexA antibody can be combined with LexA protein as a positive control

Additional information

Additional information This product can be used in:
  • studies of SOS regulation in E.coli
    detection of bait constructs in yeast two-hybrid system
  • immunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)
  • immunoprecipiation and ChIP

Related products


Background LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.

Alternative names: exrA, spr, tsl, umuA

Product citations

Selected references Hishida et al (2004) Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004 Aug 1;18(15):1886-97. doi: 10.1101/gad.1223804. PMID: 15289460; PMCID: PMC517408.

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