Name:
Phone:
E-mail:
Address:


 


LexA | LexA repressor

AS21 4541 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
LexA | LexA repressor in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4541)

DATA SHEET IN PDF

Qty: 
330
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Full-size highly-purified recombinant LexA protein, UniProt: P0A7C2
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid, containing 0.05% sodium azide
Quantity 50 µl
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)
Recommended dilution 1: 1000 - 1: 3000 (WB)
Expected | apparent MW 22.3 | 23 kDa

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-LexA (E.coli) antibodies

Escherichia coli cells (1 × 10 8 cells/mL) were lysed in lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% SDS, and bacterial protease inhibitor cocktail (Sigma) and boiled followed by gel electrophoresis on 15% SDS-PAGE gels. Anti-LexA antibodies were used at 1: 1000. 

For Western blot anti-LexA antibody can be combined with LexA protein as a positive control

Additional information

Additional information This product can be used in:
  • studies of SOS regulation in E.coli
    detection of bait constructs in yeast two-hybrid system
  • immunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)
  • immunoprecipiation and ChIP

Related products

Background

Background LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.

Alternative names: exrA, spr, tsl, umuA

Product citations

Selected references Hishida et al (2004) Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004 Aug 1;18(15):1886-97. doi: 10.1101/gad.1223804. PMID: 15289460; PMCID: PMC517408.

Related products: LexA | LexA repressor

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS21 4541P |  Protein/positive control for Western blot
420 €
AS21 4542 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
315 €