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LexA | LexA repressor (protein, positive control)

AS21 4541PProtein/positive control for Western blot
LexA | LexA repressor (protein, positive control) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4541P)

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Product Information

Purity Over 90 %, SDS-PAGE
Format Liquid, 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 1 mM DTT
Quantity 20 µg
Storage Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Expected | apparent MW 22.3 | 23 kDa

Reactivity

Application examples

Application examples Purified LexA protein (positive control for Western blot)
5 and 2 µg of highly purified LexA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.

Additional information

Additional information This product can be used in:
  • Functional studies of E.coli SOS response. This product will bind to SOS box in vitro and repress the expression of the genes belonging to SOS regulation
  • Western blot as a positive control, to confirm that the bait construct is expressed in yeast two-hybrid sstem using lexA gene
  • control in ChIP in combination with anti-LexA antibodies
LexA protein is full length, highly purified (over 90 %, SDS-PAGE) provided at a concentration of 1 mg/ml estimated by BCA method. UniProt: P0A7C2

Related products

Related products AS21 4541 | Anti-LexA | LexA repressor, rabbit anitbodies

Background

Background Escherichia coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence (TACTGTATATATATACAGTA). LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As a result, the expression of genes belonging to the S

Product citations

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