LexA | LexA repressor (protein, positive control)

Name: LexA | LexA repressor (protein, positive control)
Brand: IKP
SKU: AS21 4541P
Price: 435 EUR
Availability: InStock

AS21 4541P | Protein/positive control for Western blot

LexA | LexA repressor (protein, positive control) in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4541P)

Product Information

Purity Contains 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 1 mM DTT. Over 90 % pure by SDS-PAGE.

Format Liquid

Quantity 20 µg

Storage Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Expected | apparent MW 22,3 | 23 kDa

Reactivity

Application examples

Application examples Purified LexA protein (positive control for Western blot)

5 and 2 µg of highly purified LexA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.

Additional information

Additional information This product can be used in:

Functional studies of E.coli SOS response. This product will bind to SOS box in vitro and repress the expression of the genes belonging to SOS regulation
Western blot as a positive control, to confirm that the bait construct is expressed in yeast two-hybrid sstem using lexA gene
control in ChIP in combination with anti-LexA antibodies

LexA protein is full length, highly purified (over 90 %, SDS-PAGE) provided at a concentration of 1 mg/ml estimated by BCA method. UniProt: P0A7C2

Background

Background Escherichia coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence (TACTGTATATATATACAGTA). LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As a result, the expression of genes belonging to the S

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Purity:	Contains 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 1 mM DTT. Over 90 % pure by SDS-PAGE.
Format:	Liquid
Quantity:	20 µg
storage:	Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
calculated \| apparent molecular mass [kDa]:	22,3 \| 23 kDa
Picture (footer):	5 and 2 µg of highly purified LexA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.
additional information:	This product can be used in: Functional studies of E.coli SOS response. This product will bind to SOS box in vitro and repress the expression of the genes belonging to SOS regulation Western blot as a positive control, to confirm that the bait construct is expressed in yeast two-hybrid sstem using lexA gene control in ChIP in combination with anti-LexA antibodies
additional information (application):	LexA protein is full length, highly purified (over 90 %, SDS-PAGE) provided at a concentration of 1 mg/ml estimated by BCA method. UniProt: P0A7C2
background:	Escherichia coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence (TACTGTATATATATACAGTA). LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As a result, the expression of genes belonging to the S