Rfa3/Rpa3 | Replication factor A protein 3 (Saccharomyces cerevisiae)

AS21 4551 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae

Product Information

Immunogen Recombinant Rfa3 protein from Saccharomyces cerevisiae, UniProt: P26755

Host Rabbit

Clonality Polyclonal

Purity Serum. Contains 0.05 % sodium azide.

Format Liquid

Quantity 50 µl

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW 13,8 | 15 kDa

Reactivity

Confirmed reactivity Saccharomyces cerevisiae

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-Rfa3 (yeast) antibodies

Crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer. Blocking was done with a 5 % non-fat milk for 1 h. Primary antibody was incubated at 2: 1000, 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Background

Background Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress.

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calculated \| apparent molecular mass [kDa]:	13,8 \| 15 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Recombinant Rfa3 protein from Saccharomyces cerevisiae, UniProt: P26755
Purity:	Serum. Contains 0.05 % sodium azide.
Quantity:	50 µl
recommended dilution:	1 : 2000 (WB)
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
background:	Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress.
Confirmed reactivity:	Saccharomyces cerevisiae
predicted reactivity:	Species of your interest not listed? Contact us
Picture (footer):	Crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer. Blocking was done with a 5 % non-fat milk for 1 h. Primary antibody was incubated at 2: 1000, 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.