Rfa3/Rpa3 | Replication factor A protein 3 (Saccharomyces cerevisiae)
AS21 4551 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae

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Product Information
Immunogen
Recombinant Rfa3 protein from Saccharomyces cerevisiae, UniProt: P26755
Host
Rabbit
Clonality
Polyclonal
Purity
Serum. Contains 0.05 % sodium azide.
Format
Liquid
Quantity
50 ĩl
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
13,8 | 15 kDa
Reactivity
Confirmed reactivity
Saccharomyces cerevisiae
Predicted reactivity
Species of your interest not listed? Contact us
Application examples
Application examples

Crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer. Blocking was done with a 5 % non-fat milk for 1 h. Primary antibody was incubated at 2: 1000, 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer. Blocking was done with a 5 % non-fat milk for 1 h. Primary antibody was incubated at 2: 1000, 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Additional information
Background
Background
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress.
Product citations
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