RAD51 | DNA repair protein RAD51 (Saccharomyces cerevisiae) (ChIP grade)
AS21 4549 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae

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Product Information
Immunogen
Purified, full length, recombinant and HIS tagged RAD51 protein from Saccharomyces cerevisiae, UniProt: P25454
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4. Contains 50 % glycerol, filter sterilized.
Format
Liquid
Quantity
100 ĩg
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1: 500 : 2000 (WB)
Expected | apparent MW
43 kDa
Reactivity
Confirmed reactivity
Saccharomyces cerevisiae
Predicted reactivity
Species of your interest not listed? Contact us
Application examples
Application examples

Crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer, following blocking with 5 % non-fat milk for 1 h/RT. Primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Samples: Recombinant Rad51 protein of Saccharomyces cerevisiae, analyzed by SDS-PAGE and visualized by Coomasie stain (1), 10 ng of recombinant Rad51 protein of Saccharomyces cerevisiae (2), crude extract of Saccharomyces cerevisiae strain BY4741 (3) were separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 1000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

Crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer, following blocking with 5 % non-fat milk for 1 h/RT. Primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Samples: Recombinant Rad51 protein of Saccharomyces cerevisiae, analyzed by SDS-PAGE and visualized by Coomasie stain (1), 10 ng of recombinant Rad51 protein of Saccharomyces cerevisiae (2), crude extract of Saccharomyces cerevisiae strain BY4741 (3) were separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 1000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
Additional information
Additional information
ChIP application is described in Ribeyre and Shore 2012 while immunofluorescence is described in Muramoto et al 218.
Background
Background
S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl, Rad55, Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes.
Product citations
Selected references
Muramoto et al. (2018) Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks. Nat Commun. 2018 May 18;9(1):1995. doi: 10.1038/s41467-018-04256-y. PMID: 29777105; PMCID: PMC5959919. (IF application)
Ribeyre & Shore (2012). Anticheckpoint pathways at telomeres in yeast. Nat Struct Mol Biol. 2012 Feb 12;19(3):307-13. doi: 10.1038/nsmb.2225. PMID: 22343724. (ChIP application)
Ribeyre & Shore (2012). Anticheckpoint pathways at telomeres in yeast. Nat Struct Mol Biol. 2012 Feb 12;19(3):307-13. doi: 10.1038/nsmb.2225. PMID: 22343724. (ChIP application)
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