RAD51 | DNA repair protein RAD51 (Saccharomyces cerevisiae) (ChIP grade)

AS21 4549 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae

Product Information

Immunogen Purified, full length, recombinant and HIS tagged RAD51 protein from Saccharomyces cerevisiae, UniProt: P25454

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4. Contains 50 % glycerol, filter sterilized.

Format Liquid

Quantity 100 µg

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1: 500 : 2000 (WB)

Expected | apparent MW 43 kDa

Reactivity

Confirmed reactivity Saccharomyces cerevisiae

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-Rad51 (yeast) antibodies

Crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer, following blocking with 5 % non-fat milk for 1 h/RT. Primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Western blott using anti-Rad51 (yeast) antibodies

Western blott using anti-Rad51 (yeast) antibodies

Samples: Recombinant Rad51 protein of Saccharomyces cerevisiae, analyzed by SDS-PAGE and visualized by Coomasie stain (1), 10 ng of recombinant Rad51 protein of Saccharomyces cerevisiae (2), crude extract of Saccharomyces cerevisiae strain BY4741 (3) were separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 1000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Additional information ChIP application is described in Ribeyre and Shore 2012 while immunofluorescence is described in Muramoto et al 218.

Background

Background S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl, Rad55, Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes.

Product citations

Selected references Muramoto et al. (2018) Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks. Nat Commun. 2018 May 18;9(1):1995. doi: 10.1038/s41467-018-04256-y. PMID: 29777105; PMCID: PMC5959919. (IF application)
Ribeyre & Shore (2012). Anticheckpoint pathways at telomeres in yeast. Nat Struct Mol Biol. 2012 Feb 12;19(3):307-13. doi: 10.1038/nsmb.2225. PMID: 22343724. (ChIP application)

Confirmed reactivity:	Saccharomyces cerevisiae
predicted reactivity:	Species of your interest not listed? Contact us
additional information:	ChIP application is described in Ribeyre and Shore 2012 while immunofluorescence is described in Muramoto et al 218.
background:	S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl, Rad55, Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes.
All references:	Muramoto et al. (2018) Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks. Nat Commun. 2018 May 18;9(1):1995. doi: 10.1038/s41467-018-04256-y. PMID: 29777105; PMCID: PMC5959919. (IF application) Ribeyre & Shore (2012). Anticheckpoint pathways at telomeres in yeast. Nat Struct Mol Biol. 2012 Feb 12;19(3):307-13. doi: 10.1038/nsmb.2225. PMID: 22343724. (ChIP application)
calculated \| apparent molecular mass [kDa]:	43 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Purified, full length, recombinant and HIS tagged RAD51 protein from Saccharomyces cerevisiae, UniProt: P25454
Purity:	Immunogen affinity purified serum in PBS pH 7.4. Contains 50 % glycerol, filter sterilized.
Quantity:	100 µg
recommended dilution:	1: 500 : 2000 (WB)
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Picture (footer):	Crude extract of Saccharomyces cerevisiae was separated on a 12.5 % SDS-PAGE and blotted to a PVDF membrane using wet transfer, following blocking with 5 % non-fat milk for 1 h/RT. Primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations. Samples: Recombinant Rad51 protein of Saccharomyces cerevisiae, analyzed by SDS-PAGE and visualized by Coomasie stain (1), 10 ng of recombinant Rad51 protein of Saccharomyces cerevisiae (2), crude extract of Saccharomyces cerevisiae strain BY4741 (3) were separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 1000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

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