RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)
AS21 4550 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Purified, recombinant Rfa1 protein (without a tag) from Saccharomyces cerevisiae, UniProt: P22336
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Liquid
Quantity
100 ĩl
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunooprecipitation (IP), Western blot (WB)
Recommended dilution
1: 200 (IP), 1 : 2000 (WB)
Expected | apparent MW
70,3 kDa
Reactivity
Confirmed reactivity
Saccharomyces cerevisiae
Predicted reactivity
Species of your interest not listed? Contact us
Application examples
Application examples

10 µg of a crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 10-20 % gradient gel by SDS-PAGE and blotted to a PVDF membrane using wet transfer. Primary antibody was incubated at 1: 2000 for 1h/RT in TBS-T, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

10 µg of a crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 10-20 % gradient gel by SDS-PAGE and blotted to a PVDF membrane using wet transfer. Primary antibody was incubated at 1: 2000 for 1h/RT in TBS-T, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
Additional information
Background
Background
Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Product citations
Related products: RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €
AS21 4549 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Sacchar...
331 €
AS21 4551 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyce...
331 €