RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)

Product no: AS21 4550

AS21 4550  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Saccharomyces cerevisiae

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  • Product Info
  • Immunogen: Purified, recombinant Rfa1 protein (without a tag) from Saccharomyces cerevisiae, UniProt: P22336
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 µl
    Storage: Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunooprecipitation (IP), Western blot (WB)
    Recommended dilution: 1: 200 (IP), 1 : 2000 (WB)
    Expected | apparent MW: 70,3 kDa
  • Reactivity
  • Confirmed reactivity: Saccharomyces cerevisiae
    Predicted reactivity: Species of your interest not listed? Contact us
  • Application Examples
  • Western blot using anti-Rfa1 (yeast) antibodies

    10 µg of a crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 10-20 % gradient gel by SDS-PAGE and blotted to a PVDF membrane using wet transfer. Primary antibody was incubated at 1: 2000 for 1h/RT in TBS-T, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
  • Background
  • Background: Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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