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RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)

AS21 4550  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Saccharomyces cerevisiae

RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae) in the group Antibodies Other Species / Fungi at Agrisera AB (Antibodies for research) (AS21 4550)



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Product Information

Immunogen Purified, recombinant Rfa1 protein (without a tag) from Saccharomyces cerevisiae, UniProt: P22336
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 ĩl
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunooprecipitation (IP), Western blot (WB)
Recommended dilution 1: 200 (IP), 1 : 2000 (WB)
Expected | apparent MW 70,3 kDa

Reactivity

Confirmed reactivity Saccharomyces cerevisiae
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-Rfa1 (yeast) antibodies

10 µg of a crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 10-20 % gradient gel by SDS-PAGE and blotted to a PVDF membrane using wet transfer. Primary antibody was incubated at 1: 2000 for 1h/RT in TBS-T, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Related products

Background

Background Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

Product citations

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