RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)
AS21 4550 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae
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10 µg of a crude extract of Saccharomyces cerevisiae strain BY4741 was separated on a 10-20 % gradient gel by SDS-PAGE and blotted to a PVDF membrane using wet transfer. Primary antibody was incubated at 1: 2000 for 1h/RT in TBS-T, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
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