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Rhamnogalacturonan-Ia (clone CCRC-M2)

AS16 3134-1ml  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Cyamopsis tetragonoloba

From the laboratory of Michael G. Hahn, PhD, University of Georgia

Rhamnogalacturonan-Ia (clone CCRC-M2) in the group Antibodies Plant/Algal / Cell Wall / Dr. Hahn collection, University of Georgia at Agrisera AB (Antibodies for research) (AS16 3134-1ml)
Rhamnogalacturonan-Ia (clone CCRC-M2)



DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen MeBSA-conjugated sycamore rhamnogalacturonan-I (non-covalent),
Host Mouse
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution Undiluted or at 1 : 10 (ELISA), (IF), (IHC)

Reactivity

Confirmed reactivity Acer pseudoplatanus, Arabidopsis thaliana, Cyamopsis tetragonoloba
Predicted reactivity Rhamnogalacturonan-I from gum karaya, sycamore and Arabidopsis pectic polysaccharides
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution needs to be determined by end user

Related products

Background

Background Rhamnogalacturonans (RGs) are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:
α-D-GalpA-(1,2)-α-L-Rhap-(1)

Product citations

Selected references Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
immunogen: MeBSA-conjugated sycamore rhamnogalacturonan-I (non-covalent),
Sub class: IgM
Host: Mouse
Clonality: Monoclonal
Purity: Cell culture supernatant.
Format: Liquid
Quantity: 1 ml
storage: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
recommended dilution: Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
Confirmed reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Cyamopsis tetragonoloba
predicted reactivity: Rhamnogalacturonan-I from gum karaya, sycamore and Arabidopsis pectic polysaccharides
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: Exact working dilution needs to be determined by end user
background: Rhamnogalacturonans (RGs) are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:
α-D-GalpA-(1,2)-α-L-Rhap-(1)
All references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.