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RIQ1 | Reduced induction of non-photochemical quenching 1

AS15 2835  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

RIQ1 | Reduced induction of non-photochemical quenching 1 in the group Antibodies for Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS15 2835)

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Product Information

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana RIQ1, UniProt: Q8VYV1, TAIR: AT5G08050
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity

50 µg

Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

16 | 16 kDa (without transit peptide 10.02 kDa)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Higher plants
Species of your interest not listed? Contact us
Not reactive in cyanobacteria

Application examples

Application examples Application example


western blot using anti-RIQ1 antibodies


10 μg of total protein from Arabidopsis thaliana greening seeds under germination, were extracted and separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 from Agrisera ) diluted to 1:10 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands

Additional information

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Background

Background RIQ1 is involved in regulation of thylakoid structure. This protein has a ubiquinone oxidoreductase domain and have an electron transfer motif. 

Product citations

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