RIQ1 | Reduced induction of non-photochemical quenching 1
AS15 2835 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana RIQ1, UniProt: Q8VYV1, TAIR: AT5G08050
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
16 | 16 kDa (without transit peptide 10,02 kDa)
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Higher plants
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
cyanobacteria
Application examples
Application examples
Application example
10 μg of total protein from Arabidopsis thaliana greening seeds under germination, were extracted and separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 from Agrisera ) diluted to 1:10 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 minute.
Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands
10 μg of total protein from Arabidopsis thaliana greening seeds under germination, were extracted and separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 from Agrisera ) diluted to 1:10 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 minute.
Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands
Additional information
Background
Background
RIQ1 is involved in regulation of thylakoid structure. This protein has a ubiquinone oxidoreductase domain and have an electron transfer motif.
Product citations
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