Name:
Phone:
E-mail:
Address:






RIQ1 | Reduced induction of non-photochemical quenching 1

AS15 2835  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

RIQ1 | Reduced induction of non-photochemical quenching 1 in the group Antibodies Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS15 2835)
RIQ1 | Reduced induction of non-photochemical quenching 1



DATA SHEET IN PDF

Qty: 
302
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 132.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana RIQ1, UniProt: Q8VYV1, TAIR: AT5G08050
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 16 | 16 kDa (without transit peptide 10,02 kDa)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Higher plants
Species of your interest not listed? Contact us
Not reactive in cyanobacteria

Application examples

Application examples Application example


western blot using anti-RIQ1 antibodies


10 μg of total protein from Arabidopsis thaliana greening seeds under germination, were extracted and separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 from Agrisera ) diluted to 1:10 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands

Additional information

Related products

Background

Background RIQ1 is involved in regulation of thylakoid structure. This protein has a ubiquinone oxidoreductase domain and have an electron transfer motif. 

Product citations

immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana RIQ1, UniProt: Q8VYV1, TAIR: AT5G08050
Reconstitution: For reconstitution add 50 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]: 16 | 16 kDa (without transit peptide 10,02 kDa)
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Higher plants
Species of your interest not listed? Contact us
not reactive in: cyanobacteria
Picture (footer): Application example


western blot using anti-RIQ1 antibodies


10 μg of total protein from Arabidopsis thaliana greening seeds under germination, were extracted and separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 from Agrisera ) diluted to 1:10 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands
background: RIQ1 is involved in regulation of thylakoid structure. This protein has a ubiquinone oxidoreductase domain and have an electron transfer motif. 

Related products: RIQ1 | Reduced induction of non-photochemical quenching 1

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volum...
From 16 €
AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until...
206 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €