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Rnr1 | Ribonucleoside-diphosphate reductase large subunit (Affinity Purified)

AS21 4608   | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Saccharomyces cerevisiae

Rnr1 | Ribonucleoside-diphosphate reductase large subunit (Affinity Purified)
Rnr1 | Ribonucleoside-diphosphate reductase large subunit (Affinity Purified)



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Product Information

Immunogen Two KLH-conjugated synthetic peptides derived from c-terminal of Saccharomyces cerevisiae Rnr1 protein, sequence UniProt: P21524
Host

Rabbit

Clonality

Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 µl, of sterile or deionized water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 99.56 | 100 kDa

Reactivity

Confirmed reactivity Saccharomyces cerevisiae
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example





10 ul of total protein from  1 x 108 cells of Saccharomyces cerevisiae, extracted with lysis buffer ( 20 mM Tris, pH 8, 50 mM Ammonium acetate, 2 mM EDTA, plus protease and phosphatase inhibitor cocktails) containing 10% TCA, and re-suspended and denatured in 1X Laemmli buffer for 10 min, were separated on 10% SDS-PAGE and blotted on to nitrocellulose membrane (0.45µm) for 1.5h at constant 0.5Ampere current using wet transfer. Blots were blocked (5% milk) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 o/n at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated) diluted to 1:5000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed with Supersignal West pico chemiluminescent detection reagents (Thermo scientific). Exposure time was 1 minute.

Additional information

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Background

Background Saccharomyces cerevisiae Rnr1 (EC=1.17.4.1) is an enzyme from ribonucleoside diphosphate reductase  large chain family. Is localized to cytoplasm and provides precursors necessary for DNA synthesis. Alternative names: ribonucleotide reductase large subunit 1, ribonucleotide reductase R1 subunit 1

Product citations

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