1

Rnr1 | Ribonucleoside-diphosphate reductase large subunit (Affinity Purified)
AS21 4608 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae
- Product Info
-
Immunogen: Two KLH-conjugated synthetic peptides derived from c-terminal of Saccharomyces cerevisiae Rnr1 protein, sequence UniProt: P21524 Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: 99.56 | 100 kDa - Reactivity
-
Confirmed reactivity: Saccharomyces cerevisiae Predicted reactivity: Species of your interest not listed? Contact us Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
-
Application example
10 ul of total protein from 1 x 108 cells of Saccharomyces cerevisiae, extracted with lysis buffer ( 20 mM Tris, pH 8, 50 mM Ammonium acetate, 2 mM EDTA, plus protease and phosphatase inhibitor cocktails) containing 10% TCA, and re-suspended and denatured in 1X Laemmli buffer for 10 min, were separated on 10% SDS-PAGE and blotted on to nitrocellulose membrane (0.45µm) for 1.5h at constant 0.5Ampere current using wet transfer. Blots were blocked (5% milk) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 o/n at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated) diluted to 1:5000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed with Supersignal West pico chemiluminescent detection reagents (Thermo scientific). Exposure time was 1 minute.
- Background
-
Background: Saccharomyces cerevisiae Rnr1 (EC=1.17.4.1) is an enzyme from ribonucleoside diphosphate reductase large chain family. Is localized to cytoplasm and provides precursors necessary for DNA synthesis. Alternative names: ribonucleotide reductase large subunit 1, ribonucleotide reductase R1 subunit 1 - Protocols
-
Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
-
To leave a review, you should have previously purchased this product and log in to your customer profile.
This product doesn't have any reviews.
Accessories

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer
AS16 ECL-SN, 100 ml
AS16 ECL-SN-1L, 1L
Choose the appropriate volume in the drop down menu to the right