RPS6A | 40S ribosomal protein S6-1
AS19 4292 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana RPS6A, UniProt: O48549, TAIR: At4g31700
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
28,3 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Actinidia rufa, Ananas comosus, Beta vulgaris, Cajanus cajan, Capsicum chinense, Citrus clementina, Gossypium australe, Olea europaea subsp. europaea, Oryza sativa, Panicum miliaceum, Populus alba, Sesamum indicum, Senna tora, Solanum lycopersicum, Zea mays, Vigna unguiculata
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Chlamydomonas reinhardtii
Application examples
Application examples

0.5 µg/well of total protein extracted freshly from Arabidopsis thaliana whole leaf with extraction buffer ( 50mM Tris-HCl pH 7.5, 1mM NaCl, 1% Triton X-100, 1mM DTT, 1mM PMSF, 0.5mM EDTA and 1X Halt protease and phosphatase inhibitor cocktail) and denatured with laemmli sample buffer at 70°C for 5 min. Protein extract were separated on 4-12% SDS-PAGE and blotted 1h to nitrocellulose (pore size of 0.45um), using wet transfer. Blot was blocked with 5% BSA for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in 5% BSA+PBS-T ON/4°C with agitation. The antibody solution was decanted and the blot rinsed, then washed 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in 5% BSA+PBS-T for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 10 seconds.
Courtesy Dr. Irabonosi Obomighie, University of Essex, United Knigdom

0.5 µg/well of total protein extracted freshly from Arabidopsis thaliana whole leaf with extraction buffer ( 50mM Tris-HCl pH 7.5, 1mM NaCl, 1% Triton X-100, 1mM DTT, 1mM PMSF, 0.5mM EDTA and 1X Halt protease and phosphatase inhibitor cocktail) and denatured with laemmli sample buffer at 70°C for 5 min. Protein extract were separated on 4-12% SDS-PAGE and blotted 1h to nitrocellulose (pore size of 0.45um), using wet transfer. Blot was blocked with 5% BSA for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in 5% BSA+PBS-T ON/4°C with agitation. The antibody solution was decanted and the blot rinsed, then washed 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in 5% BSA+PBS-T for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 10 seconds.
Courtesy Dr. Irabonosi Obomighie, University of Essex, United Knigdom
Additional information
Background
Background
RPS6A (40S ribosomal protein S6-1) is the major substrate of protein kinases in eukaryote ribosomes. Essential during gametogenesis.
Product citations
Selected references
To be added when available, antibody released in November 2020.
Related products: RPS6A | 40S ribosomal protein S6-1
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
Now available only...
Now available only...
From 26 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €
AS19 4291 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis ...
367 €
AS19 4302 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thali...
367 €