RPS6A | 40S ribosomal protein S6-1

Product no: AS19 4292

AS19 4292   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana RPS6A, UniProt: O48549, TAIR: At4g31700
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 28.3 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Actinidia rufa, Ananas comosus, Beta vulgaris, Cajanus cajan, Capsicum chinense, Citrus clementina, Gossypium australe, Olea europaea subsp. europaea, Oryza sativa, Panicum miliaceum, Populus alba, Sesamum indicum, Senna tora, Solanum lycopersicum, Solanum tuberosum, Zea mays, Vigna unguiculata

    Species of your interest not listed? Contact us
    Not reactive in: Chlamydomonas reinhardtii
  • Application Examples

  • Western blot using anti-RPS6A antibodies

    0.5 µg/well of total protein extracted freshly from Arabidopsis thaliana whole leaf with extraction buffer ( 50mM Tris-HCl pH 7.5, 1mM NaCl, 1% Triton X-100, 1mM DTT, 1mM PMSF, 0.5mM EDTA and 1X Halt protease and phosphatase inhibitor cocktail) and denatured with laemmli sample buffer at 70°C for 5 min. Protein extract were separated on  4-12% SDS-PAGE  and blotted 1h to nitrocellulose (pore size of   0.45um), using wet transfer. Blot was blocked with 5% BSA for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in 5% BSA+PBS-T ON/4°C with agitation. The antibody solution was decanted and the blot rinsed, then washed 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in 5% BSA+PBS-T for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 10 seconds.

    Courtesy Dr. Irabonosi Obomighie, University of Essex, United Knigdom
  • Background
  • Background: RPS6A  (40S ribosomal protein S6-1)  is the major substrate of protein kinases in eukaryote ribosomes. Essential during gametogenesis.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS19 4291   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

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