Name:
Phone:
E-mail:
Address:


 


RPS6A-P240 | Phosphorylated (Ser240) 40S ribosomal protein S6-1

AS19 4302   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

RPS6A-P240 | Phosphorylated (Ser240) 40S ribosomal protein S6-1 in the group Antibodies for Plant/Algal  / Developmental Biology / Circadian clock at Agrisera AB (Antibodies for research) (AS19 4302)

DATA SHEET IN PDF

Qty: 
355
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana RPS6A, UniProt: O48549, TAIR: At4g31700 phosphorylated at Ser240
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Actinidia rufa, Ananas comosus, Beta vulgaris, Cajanus cajan, Capsicum chinense, Citrus clementina, Gossypium australe, Olea europaea subsp. europaea, Oryza sativa, Panicum miliaceum, Populus alba, Sesamum indicum, Senna tora, Solanum lycopersicum, Vigna unguiculata

Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-RPS6A/P240 antibodies

Left panel is developed with anti-RPS6-p240,  while right panel anti-RPS6, which serves as a loading control.


Lanes: 

1 and 4 - 0.5 µg of Arabidopsis thaliana whole leaf extract from untreated leaf discs

2 and 5 - 0.5 µg of Arabidopsis thaliana whole leaf extract from leaf discs treated with 10 mM glutamine (TOR activator) for 8 hours

3 and 6 - 0.5 µg of Arabidopsis thaliana whole leaf extract from leaf discs treated with 2 µM AZD-8055 (TOR inhibitor) for 8 hours


0.5 µg/well of total protein extracted freshly from mature Arabidopsis thaliana leaves  with 50 mM HEPES pH 7.5, 5mM NaF, 2.5 mM NaPPi, 25 mM B-phosphoglycerol, Roche Complete inhibitor tablet (1x), 2% PVPP, 2 mM PMSF) and denatured with 2x SDS sample buffer (80 mM tris pH 6.8, 2% SDS, 10% glycerol, 100 mM DTT, bromophenol blue) at 99°C for 3 min. were separated on AnyKd (BioRAD) gradient % SDS-PAGE  and blotted 1h to PVDF (pore size of  0.45  um) using semi-dry transfer. Blot was blocked with 2% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in TBS-T + 2% milk powder ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in TBS-T + 2 % milk powder  for 1h/RT with agitation. The blot was washed as above and developed for 5  min with Agrisera ECLBright with  GE Amersham Imager 600. Exposure time was ~60.seconds.

Courtesy of Dr. Brendan O'Leary, University of Western Australia, School of Molecular Sciences, ARC Centre for Plant Energy Biology, Australia

Additional information

Related products

Background

Background RPS6A  (40S ribosomal protein S6-1)  is the major substrate of protein kinases in eukaryote ribosomes. Essential during gametogenesis.

Product citations

Selected references To be added when available, antibody released in February 2021.

Related products: RPS6A-P240 | Phosphorylated (Ser240) 40S ribosomal protein S6-1

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Off...
194 €
AS19 4292   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thali...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS19 4291   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis ...
355 €