Sheep anti-Rabbit IgG (H&L), HRP conjugated

Product no: AS10 1538
AS10 1538 | Clonality: Polyclonal | Host: Sheep | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

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  • Product Info
  • Immunogen: Purified rabbit IgG (H&L)
    Host: Sheep
    Clonality: Polyclonal
    Purity: Immunogen affinity purified sheep IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
  • Additional Information
  • Additional information:

    Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.

    Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.

  • Background
  • Background: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
  • Protocols
  • Antibody protocols
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