SOBIR1 | Suppressor of BIR1 (chicken antibody)

AS16 3959 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana

Product Information

Immunogen KLH-conjugated peptide, chosen from Arabidopsis thaliana SOBIR1 sequence, UniProt: Q9SKB2, TAIR:At2g31880

Host Chicken

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Co-Immunoprecipitation (co-IP), Western blot (WB)

Recommended dilution 1: 1000 (WB)

Expected | apparent MW 71 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamiana

Predicted reactivity
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Not reactive in

Application examples

Additional information

Antibody is recognizing AtSOBIR1, SISOBIR1 and endogenous NbSOBIR1.

In co-IP experiment one band of expeceted size is observed for NbSOBIR1 (72 kDa), AtSOBIR1-Myc(86 kDa) and SISOBIR1-Myc (84 kDa)

Related products

AS16 3204 | Anti-SOBIR1 | Suppressor of BIR1, rabbit antibodies

AS12 1859 | Anti-BRI1 | BRASSINOSTEROID INSENSITIVE 1, rabbit antibodies

AS12 1857 | Anti-FLS2 | FLAGELLIN-SENSITIVE 2, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

SOBIR1 is a kinase with dual specificity acting on both serine/threonine and tyrosine-containing substrates. It is involved in the activation of plant defense and cell death. It also regulates abscission.