Sporamin

AS10 697 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Ipomoea batatas


product information
Background		Sporamin is a storage protein of tuberous roots of sweet potato and accounts for more than 80 % of the soluble protein content. Protein is localized to vacuole.
Immunogen		native protein purified from sweet potato taproot
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Total IgG
Format		Lyophilized
Quantity		100 µl
Reconstitution		For reconstitution add 100 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		antibodies to vacuolar proteins Plant protein extraction buffer Secondary antibodies
Additional information		Contains less than 0.1 % sodium azide as preservative

application information
Recommended dilution		1 : 2000 (WB)
Expected \| apparent MW		25 kDa (when fresh DTT is used in sample buffer)
Confirmed reactivity		Hordeum vulgare
Predicted reactivity		Ipomoea sp.
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody is recognizing both, sporamin A and B.
Selected references		Maeshima et al. (1985) Characterization of major proteins in sweet potato tuberous roots. Phytochemistry, 24: 1899-1902. Hattori et al. (1985) Molecular cloning and nucleotide sequence of cDNA for sporamin, the major soluble protein of sweet potato tuberous roots. Plant Molecular Biology, 5: 313-320.

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