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STM | Homeobox protein SHOOT MERISTEMLESS

AS11 1764 | Clonality: Polyclonal | Host: Rabbit | predicted Reactivity: A. thaliana

STM | Homeobox protein SHOOT MERISTEMLESS in the group Plant/Algal Antibodies / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS11 1764)

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product information
Background

Homeobox protein SHOOT MERISTEMLESS is a protein required for shoot apical meristem (SAM) formation during embryogenesis.

Immunogen

KLH-conjugated peptide chosen from  Arabidopsis thaliana STM protein sequence, UniProt: Q38874,TAIR: At1g62360

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies for developmental biology

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

42.7 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in June 2015


application example

western blot using anti-STM antibodies

 

10 µg of total protein from Arabidopsis thaliana seedling tissue extracted with Exractions buffers 1 or 2 (see below) were separated on 10 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in milk overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed twice with milk, then washed once for 5 mins with PBS, 10 mins with 2% milk + PBST, 5 mins with PBST at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:75 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was variable.
Extraction buffers:
1. 50mM tris-acetate pH 7.9 100mM potassium acetate 20% glycerol 1mM EDTA 1mM DTT 1x Roche Protease inhibitor cocktail
2.  100mM tris-cl pH 7.5 75mM Nacl 15mM EGTA 15mM MgCl2 1mM DTT 400 mM B glycophosphate 1x protease inhibitor cocktail NaFl, Na pyrophosphate, Na vanadate.

Courtesy Dr. Simon Scofield, Cardiff University, UK

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