STM | Homeobox protein SHOOT MERISTEMLESS
AS11 1764 | Clonality: Polyclonal | Host: Rabbit | predicted Reactivity: A. thaliana

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Product Information
Immunogen
KLH-conjugated peptide chosen from Arabidopsis thaliana STM protein sequence, UniProt: Q38874,TAIR: At1g62360
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
42,7 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brassica napus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
Extraction buffers:
1. 50mM tris-acetate pH 7.9 100mM potassium acetate 20% glycerol 1mM EDTA 1mM DTT 1x Roche Protease inhibitor cocktail
2. 100mM tris-cl pH 7.5 75mM Nacl 15mM EGTA 15mM MgCl2 1mM DTT 400 mM B glycophosphate 1x protease inhibitor cocktail NaFl, Na pyrophosphate, Na vanadate.
Courtesy Dr. Simon Scofield, Cardiff University, UK
10 µg of total protein from Arabidopsis thaliana seedling tissue extracted with Exractions buffers 1 or 2 (see below) were separated on 10 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in milk overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed twice with milk, then washed once for 5 mins with PBS, 10 mins with 2% milk + PBST, 5 mins with PBST at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:75 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was variable.
Extraction buffers:
1. 50mM tris-acetate pH 7.9 100mM potassium acetate 20% glycerol 1mM EDTA 1mM DTT 1x Roche Protease inhibitor cocktail
2. 100mM tris-cl pH 7.5 75mM Nacl 15mM EGTA 15mM MgCl2 1mM DTT 400 mM B glycophosphate 1x protease inhibitor cocktail NaFl, Na pyrophosphate, Na vanadate.
Courtesy Dr. Simon Scofield, Cardiff University, UK
Additional information
Background
Background
Homeobox protein SHOOT MERISTEMLESS is a protein required for shoot apical meristem (SAM) formation during embryogenesis.
Product citations
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