STT7 | Serine/threonine-protein kinase STT7 (chloroplastic)
|Data sheet||Product citations||Customer reviews|
Total cells of Chlamydomonas reinhardtii were kept under state I (no expression of LHCII kinase, line 1) and state II (expression of LHCII kinase, line 2). 2 µg of chlorophyll content were solubilised in buffer containing 120 mM Tris (6.8) 4% SDS, 20% glycerol and 1 mM DTT and denatured with at 100°C for 1 min (Takahashi et al., 2013) were separated on 12 % SDS-PAGE and blotted 20 min to nitrocellulose using semi-dry transfer. Blot was blocked with 4% milk RT/2h with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 for TBS-T ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for 30 sec with ECL. Exposure time was 30 to 60 seconds. The molecular weight of the identified band is approx. 80 KD.
Courtesy Dr. Sai Kiran, University of Hyderbad, India
Upadhyaya and Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.
Lameille et al. (2009). Analysis of the chloroplast protein kinase Stt7 during state transitions. PLoS Biol. 2009 Mar 3;7(3):e45. doi: 10.1371/journal.pbio.1000045.
Related products: STT7 | Serine/threonine-protein kinase STT7 (chloroplastic)
This product can b...