Anti-T7 Tag (monoclonal, clone 8E11)

Name: Anti-T7 Tag (monoclonal, clone 8E11)
SKU: AS25 5101
Price: 169 EUR
Availability: InStock

Product no: AS25 5101

AS25 5101 | Clonality: Monoclonal | Host: Mouse | Reactivity: T7-tagged proteins

169 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Sub class:	IgG
Immunogen:	Carrier conjugated synthetic peptide T7
Host:	Mouse
Clonality:	Monoclonal
Purity:	Affinity purified in PBS pH 7.4 and 50 % glycerol. Contains 0.02 % sodium azide.
Format:	Liquid
Quantity:	50 µg
Storage:	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	ELISA (ELISA), Western blot (WB)
Recommended dilution:	1:500-1:5000 (WB)
Expected \| apparent MW:	Depends upon MW of a fusion partner

Reactivity
Confirmed reactivity: T7 epitope tagged proteins
Application Examples
1.5 - 0.5 μg of T7 fusion protein/well. Primary antibody was used in dilution 1: 3000.
Additional Information
Additional information (application): Antibody is present in PBS, pH 7.4, containing 0.5% BSA and 50% Glycerol. Contains 0.02% sodium azide as preservative.
Background

Background:

The T7 tag is a short peptide sequence derived from the T7 bacteriophage gene 10. It is commonly used as an epitope tag in recombinant protein expression systems. The T7 tag facilitates the detection and purification of tagged proteins, including antibodies, due to its high specificity and strong binding affinity to anti-T7 tag antibodies.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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