TRITC | tetramethylrhodamine isothiocyanate, HRP-conjugated (monoclonal, Clone NI 308)
AS18 4226 | Clonality: Monoclonal | Host: Mouse | Reactivity: TRITC molecule
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Product Information
Immunogen
Highly purified tetramethylrhodamine isothiocyanate isomer R.
Host
Mouse
Clonality
Monoclonal
Purity
Purified mouse IgG1 kappa in phosphate buffered saline (pH 7.2)
Format
Lyophilized
Quantity
200 µg
Reconstitution
For reconstitution add 0.5 ml of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Stort time storage at +4°C.
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Dot blot (Dot), Western blot (WB)
Recommended dilution
The optimum working dilution should be determined by titration by end user.
1:100 (ELISA), 1:10-1:40 (IHC)
1:100 (ELISA), 1:10-1:40 (IHC)
Reactivity
Confirmed reactivity
Tetramethylrhodamine isothiocyanate isomer R
Application examples
Additional information
Additional information
This product is intended FOR RESEARCH USE ONLY.
This product has not been tested for cross-reactivity with other fluorescent compounds.
Background
Background
TRITC is a bright orange-fluorescent dye. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This antibody is used to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC.
Product citations
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Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
