TRITC | tetramethylrhodamine isothiocyanate (monoclonal, Clone NI 308)

AS18 4225 | Clonality: Monoclonal | Host: Mouse | Reactivity: TRITC molecule

Product Information

Immunogen Highly purified tetramethylrhodamine isothiocyanate isomer R,

Host Mouse

Clonality Monoclonal

Purity Purified mouse IgG.1 kappa in PBS pH 7.2.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 0,5 ml of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.

Tested applications ELISA (ELISA), Indirekt Immunoflourescense (IIF)

Recommended dilution The optimum working dilution should be determined by titration by end user.
1:100 (ELISA), 1:10-1:40 (IIF)

Reactivity

Confirmed reactivity Tetramethylrhodamine isothiocyanate isomer R

Application examples

Additional information

Additional information This product is intended FOR RESEARCH USE ONLY

This product has not been tested for cross-reactivity with other fluorescent compounds

Background

TRITC is a bright orange-fluorescent dye. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This antibody is used to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC.

Product citations

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Clonality:	Monoclonal
Format:	Lyophilized
Host:	Mouse
immunogen:	Highly purified tetramethylrhodamine isothiocyanate isomer R,
Purity:	Purified mouse IgG.1 kappa in PBS pH 7.2.
Quantity:	200 µg
recommended dilution:	The optimum working dilution should be determined by titration by end user. 1:100 (ELISA), 1:10-1:40 (IIF)
Reconstitution:	For reconstitution add 0,5 ml of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
tested applications:	ELISA (ELISA), Indirekt Immunoflourescense (IIF)
Confirmed reactivity:	Tetramethylrhodamine isothiocyanate isomer R
background:	TRITC is a bright orange-fluorescent dye. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This antibody is used to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC.
additional information:	This product is intended FOR RESEARCH USE ONLY
additional information (application):	This product has not been tested for cross-reactivity with other fluorescent compounds