Urease

AS09 559 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Canavalia ensiformis (common jack-bean)

20 % until end of March 2023. Use discount code: Nitro20

Product Information

Immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Recommended dilution 1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

91 kDa

Reactivity

Confirmed reactivity Canavalia ensiformis

Predicted reactivity Arabidopsis thaliana, Glycine max, Ricinus communis
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

Product citations

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Confirmed reactivity:	Canavalia ensiformis
predicted reactivity:	Arabidopsis thaliana, Glycine max, Ricinus communis Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
additional information:	Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
calculated \| apparent molecular mass [kDa]:	91 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	urease isolated and purified from Canavalia ensiformis (common jack-beans)
Purity:	Purified IgG in PBS.
Quantity:	10 mg
recommended dilution:	1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)