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Urease, Biotin conjugated

AS09 560 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Canavalia ensiformis (common jack-bean)

Urease, Biotin conjugated in the group Antibodies for Plant/Algal  / Nitrogen Metabolism at Agrisera AB (Antibodies for research) (AS09 560)

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Product Information

Immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

Host Rabbit
Clonality Polyclonal
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Recommended dilution 1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

91 kDa

Reactivity

Confirmed reactivity Canavalia ensiformis
Predicted reactivity Arabidopsis thaliana, Glycine max, Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Related products

Related products

AS09 559 | anti-urease

AS09 561 | anti-urease (affinity purified)

Plant protein extraction buffer

Background

Background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

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Selected references

to be added when available