Urease, Biotin conjugated

AS09 560 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Canavalia ensiformis (common jack-bean)

Product Information

Immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Recommended dilution 1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

91 kDa

Reactivity

Confirmed reactivity Canavalia ensiformis

Predicted reactivity Arabidopsis thaliana, Glycine max, Ricinus communis
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Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Related products

AS09 559 | Anti-urease, rabbit antibodies

AS09 561 | Anti-urease (affinity purified), rabbit antibodies

Plant protein extraction buffer

Background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

Product citations

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