Urease, Biotin conjugated
AS09 560 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Canavalia ensiformis (common jack-bean)
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Product Information
Immunogen
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG
Format
Lyophilized in phosphate buffer saline
Quantity
10 mg
Reconstitution
For reconstitution add 1 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Recommended dilution
1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
91 kDa
Reactivity
Confirmed reactivity
Canavalia ensiformis
Predicted reactivity
Arabidopsis thaliana, Glycine max, Ricinus communis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
Background
Background
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Product citations
Selected references
to be added when available
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