AS15 3027  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: V5-tagged fusion proteins

V5 in the group Tag Antibodies / V5 at Agrisera AB (Antibodies for research) (AS15 3027)


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product information
Background V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.


KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

Host Mouse
Clonality Monoclonal
Clone IgG1, clone V5.E10
Purity Protein A purified
Format Lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
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AS15 2964 | V5, ALP conjugated (rabbit antibody)

AS15 2965 | V5, Biotin conjugated (rabbit antibody)

AS15 2966
| V5, DyLight® 488 conjugated (rabbit antibody)

AS15 2967 | V5, DyLight® 550 conjugated (rabbit antibody)

AS15 2968 | V5, DyLight® 650 conjugated (rabbit antibody)

AS15 2969 | V5, SureLight® R-Phycoerythrin conjugated (rabbit antibody)

other tag antibodies

Secondary antibodies

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
application information
Recommended dilution 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)
Expected | apparent MW
Confirmed reactivity V5-tagged fusion proteins
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody is purified on Protein A.

For western blot incubate antibody for one hour at room temperature.
Selected references

to be added when available antibody available in October 2015

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