V5 (clone V5.E10)

AS15 3027 | Clonality: monoclonal | Host: Mouse | Reactivity: V5-tagged fusion proteins

Product Information

Immunogen

KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide

Format Liquid

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)

Reactivity

Confirmed reactivity V5-tagged fusion proteins

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally

Antibody is purified on Protein A.

For western blot incubate antibody for one hour at room temperature.

Related products

AS15 3031 | Anti-V5, mouse antibodies

AS15 2964 | Anti-V5, ALP conjugated, rabbit antibodies

AS15 2965 | Anti-V5, Biotin conjugated, rabbit antibodies

AS15 2966 | Anti-V5, DyLight® 488 conjugated, rabbit antibodies

AS15 2967 | Anti-V5, DyLight® 550 conjugated, rabbit antibodies

AS15 2968 | Anti-V5, DyLight® 650 conjugated, rabbit antibodies

AS15 2969 | Anti-V5, SureLight® R-Phycoerythrin conjugated, rabbit antibodies

other tag antibodies

Secondary antibodies

Background

Background V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

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