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V5 (clone V5,E10)

AS15 3027  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: V5-tagged fusion proteins

V5 (clone V5,E10) in the group Tag Antibodies / V5/VSV at Agrisera AB (Antibodies for research) (AS15 3027)



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How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format Liquid
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)

Reactivity

Confirmed reactivity V5-tagged fusion proteins
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
Antibody is purified on Protein A.

For western blot incubate antibody for one hour at room temperature.

Related products

Background

Background V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

Product citations

Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen:

KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide
Purity: Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Quantity: 50 µg
recommended dilution: 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)
Reconstitution: For reconstitution add 50 µl of sterile water
storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgG1
tested applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
background: V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

Confirmed reactivity: V5-tagged fusion proteins
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
additional information (application): Antibody is purified on Protein A.

For western blot incubate antibody for one hour at room temperature.

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