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V5 (clone V5,E10)

AS15 3027  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: V5-tagged fusion proteins

V5 (clone V5,E10) in the group Tag Antibodies / V5/VSV at Agrisera AB (Antibodies for research) (AS15 3027)



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format Liquid
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)

Reactivity

Confirmed reactivity V5-tagged fusion proteins
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
Antibody is purified on Protein A.

For western blot incubate antibody for one hour at room temperature.

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AS15 3031 | Anti-V5, mouse antibodies

AS15 2964 | Anti-V5, ALP conjugated, rabbit antibodies

AS15 2965 | Anti-V5, Biotin conjugated, rabbit antibodies

AS15 2966 | Anti-V5, DyLight® 488 conjugated, rabbit antibodies

AS15 2967 | Anti-V5, DyLight® 550 conjugated, rabbit antibodies

AS15 2968 | Anti-V5, DyLight® 650 conjugated, rabbit antibodies

AS15 2969 | Anti-V5, SureLight® R-Phycoerythrin conjugated, rabbit antibodies

other tag antibodies

Secondary antibodies

Background

Background V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

Product citations

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