V5 (clone V5,E10)
AS15 3027 | Clonality: monoclonal | Host: Mouse | Reactivity: V5-tagged fusion proteins

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Product Information
Immunogen
KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format
Liquid
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)
Reactivity
Confirmed reactivity
V5-tagged fusion proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
Antibody is purified on Protein A.
For western blot incubate antibody for one hour at room temperature.
For western blot incubate antibody for one hour at room temperature.
Background
Background
V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.
Product citations
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