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VPS26A | Vacuolar protein sorting 26A

AS13 2632  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Arabidopsis thaliana

VPS26A | Vacuolar protein sorting 26A in the group Plant/Algal Antibodies / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS13 2632)

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product information
Background

VPS26A (Vacuolar protein sroting 26A) is a part of a retromer-like protein complex involved in endosome to lusosome protein transport. Synonymes: vesicle protein sroting 26A.

Immunogen

Recombinant VPS26A from Arabidopsis thaliana UniProt: Q9FJD0, TAIR: (At5g53530)

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS13 2633 | anti-VPS29 | Vacuolar protein sorting 29, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

35 kDa

Confirmed reactivity Arabidopsis thaliana (protoplasts and total cell extract)
Predicted reactivity Glycne max, Picea chinensis, Populus balsamifera, Ricinus communis, Rosa rugosa, Solanum lycopersicum, Solanum tuberosum, Sorghum vulgare, Zea mays, Vitis vinifera
Not reactive in

Nicotiana tabacum

Additional information
Selected references

to be added when available, antibody released in December 2014


application example

western blot using anti-VPS26 antibodies

50 µg of total protein from Arabidopsis thaliana suspension culture (1), 50 µg of unpurified total protein from Arabidopsis thaliana (2), 50 µg of total protein from Nicotiana tabacum leaf protoplasts (3), 20 µl of unpurified total leaf extract from Nicotiana tabacum (4), extracted with buffer containing 100 mM Tris (pH 7.8), 200 mM NaCl, 1 mM EDTA, 2% (v/v) beta-mercaptoethanol and 0.2% (v/v) Triton X-100, were separated on 10% SDS-PAGE and blotted 2h to nitrocellulose (semidry blot at 200 mA and RT). Blots were blocked with 5% (w/v) milk powder and 1% (w/v) BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP conjugated) diluted to 1:10 000 in blocking solution for 45 min. at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds.

Courtesy of Simone Früholz, University of Tuebingen, Germany

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