VPS26A | Vacuolar protein sorting 26A

Product no: AS13 2632

AS13 2632  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    Recombinant VPS26A from Arabidopsis thaliana UniProt: Q9FJD0, TAIR: (At5g53530)

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5 000 (WB)
    Expected | apparent MW:

    35 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana (protoplasts and total cell extract)
    Predicted reactivity: Glycne max, Picea chinensis, Populus balsamifera, Ricinus communis, Rosa rugosa, Solanum lycopersicum, Solanum tuberosum, Sorghum vulgare, Zea mays, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: Nicotiana tabacum
  • Application Examples
  • Application example

    western blot using anti-VPS26 antibodies

    50 µg of total protein from Arabidopsis thaliana suspension culture (1), 50 µg of unpurified total protein from Arabidopsis thaliana (2), 50 µg of total protein from Nicotiana tabacum leaf protoplasts (3), 20 µl of unpurified total leaf extract from Nicotiana tabacum (4), extracted with buffer containing 100 mM Tris (pH 7.8), 200 mM NaCl, 1 mM EDTA, 2% (v/v) beta-mercaptoethanol and 0.2% (v/v) Triton X-100, were separated on 10% SDS-PAGE and blotted 2h to nitrocellulose (semidry blot at 200 mA and RT). Blots were blocked with 5% (w/v) milk powder and 1% (w/v) BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP conjugated) diluted to 1:10 000 in blocking solution for 45 min. at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds.

    Courtesy of Simone Früholz, University of Tuebingen, Germany
  • Background
  • Background:

    VPS26A (Vacuolar protein sroting 26A) is a part of a retromer-like protein complex involved in endosome to lusosome protein transport. Synonymes: vesicle protein sroting 26A.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS13 2633   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

319 €
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Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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