VPS29 | Vacuolar protein sorting 29

Product no: AS13 2633

AS13 2633   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    Recombinant VPS29 from Arabidopsis thaliana UniProt: Q9STT2, TAIR: At3g47810

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: 20,9 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana (protoplasts and total cell extract)
    Predicted reactivity: Glycne max, Medicago tribuloides, Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: Nicotiana tabacum
  • Application Examples
  • Application example


    western blot using anti-VPS29 antibodies

    50 µg of total protein from Arabidopsis thaliana suspension culture (1), 50 µg of unpurified total protein from Arabidopsis thaliana (2), 50 µg of total protein from Nicotiana tabacum leaf protoplasts (3), 20 µl of unpurified total leaf extract from Nicotiana tabacum (4), extracted with buffer containing 100 mM Tris (pH 7.8), 200 mM NaCl, 1 mM EDTA, 2% (v/v) beta-mercaptoethanol and 0.2% (v/v) Triton X-100, were separated on 10% SDS-PAGE and blotted 2h to nitrocellulose (semidry blot at 200 mA and RT). Blots were blocked with 5% (w/v) milk powder and 1% (w/v) BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP conjugated) diluted to 1:10 000 in blocking solution for 45 min. at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds.

    Courtesy of Simone Früholz, University of Tuebingen, Germany

  • Background
  • Background:

    VPS29 (vacuolar protein sorting 29) is a part of a retromer-like protein complex involved in endosome to lysosome protein transport. Synonymes:Vacuolar protein sorting-associated protein 29, Protein MAIGO 1.

  • Product Citations
  • Selected references: Munch et al. (2015). Retromer Contributes to Immunity-Associated Cell Death in Arabidopsis. Plant Cell. 2015 Feb 13. pii: tpc.114.132043.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS13 2632  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Arabidopsis thaliana

302 €
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Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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