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VPS29 | Vacuolar protein sorting 29

AS13 2633   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

VPS29 | Vacuolar protein sorting 29 in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS13 2633)

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant VPS29 from Arabidopsis thaliana UniProt: Q9STT2, TAIR: At3g47810

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

20.9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana (protoplasts and total cell extract)
Predicted reactivity Glycne max, Medicago tribuloides, Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in Nicotiana tabacum

Application examples

Application examples

Application example


western blot using anti-VPS29 antibodies

50 µg of total protein from Arabidopsis thaliana suspension culture (1), 50 µg of unpurified total protein from Arabidopsis thaliana (2), 50 µg of total protein from Nicotiana tabacum leaf protoplasts (3), 20 µl of unpurified total leaf extract from Nicotiana tabacum (4), extracted with buffer containing 100 mM Tris (pH 7.8), 200 mM NaCl, 1 mM EDTA, 2% (v/v) beta-mercaptoethanol and 0.2% (v/v) Triton X-100, were separated on 10% SDS-PAGE and blotted 2h to nitrocellulose (semidry blot at 200 mA and RT). Blots were blocked with 5% (w/v) milk powder and 1% (w/v) BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP conjugated) diluted to 1:10 000 in blocking solution for 45 min. at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 20 seconds.

Courtesy of Simone Früholz, University of Tuebingen, Germany

Additional information

Related products

Related products

AS13 2632 | Anti-VPS26A | Vacuolar protein sorting 26A, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

VPS29 (vacuolar protein sorting 29) is a part of a retromer-like protein complex involved in endosome to lysosome protein transport. Synonymes:Vacuolar protein sorting-associated protein 29, Protein MAIGO 1.

Product citations

Selected references Munch et al. (2015). Retromer Contributes to Immunity-Associated Cell Death in Arabidopsis. Plant Cell. 2015 Feb 13. pii: tpc.114.132043.

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