Anti-PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)

Justus Niemeyer
This antibody worked very well for Chlamydomonas reinhardtii. We used it for immunoblot and loaded 5-10 µg total protein. Dilution of 1:10000 for 1h is sufficient. We are satisfied and would buy again!	2020-09-15 @ 17:58:46
Soo Yeon Ko
This antibody is also good for Oryza Sativa. The samples were treated in light stress, and we could see the D1 band. We used 1:10000 dillution.	2019-11-18 @ 13:12:02
Katya Georgieva
We used the antibody to detect the protein changes of D1during desiccation of Haberlea rhodopensis plants. PsbA worked well on thylakoid proteins (3ug chlorophyll per lane, 1:20000 dilution of PsbA, ECL detection of WB). The antibody gave 2 bands due to cross-reaction with cytochrome b559. Reference: Mihailova G, Büchel C, Dietzel L, Georgieva K (2016) Desiccation induced changes in photosynthesis related proteins of shade and sun Haberlea rhodopensis plants. Compt. Rend Bulg. Acad. Sci. 69(1), 37-44	2019-06-24 @ 09:24:01
Marcin Nowik
This antibody worked fine for Arabidopsis grown in medium light, giving a band of expected size, but broad. After stress treatment, lower molecular mass degradation product was also detected. We used 1:10 000 dillution for 1h.	2016-07-26 @ 14:10:20
Dimitris Petroutsos
This antibody worked very well on whole cell extracts of Phaeodactylum tricornutum. Here is the detailed protocol we followed:4 µg of total protein from Phaeodactylum tricornutum were loaded on a 13% SDS-PAGE and blotted 1.5 h to nitrocellulose. Blots were blocked immediately following transfer in 5% milk-TBS-T for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1:10000 overnight at 4 oC with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed 6 sec with ECL detection reagent. Images of the blots were obtained using a CCD imager (Chemidock MP Imaging, Bio-Rad).	2014-12-20 @ 23:08:39
Katarzyna Hura
This antibody works well. We have tried this antibody on wheat and barley. I have been using recommended dilution 1:20 000.	2013-10-30 @ 13:16:13
Sujith Puthiyaveetil
This antibody works well. It gives clean results. I usually get a strong band around 30 kDa. Sometimes, this 30 kDa band is shifted down a little bit (around 28 kDa). In response to photoinhibition, one could also see bands around 55-60 kDa, in addition to the 28 kDa band. This could be the aggregated or cross-linked D1, as other reviewers suggest. Other than Arabidopsis, we have tried this antibody on rice, wheat, Arundo, Craterostigma, and Lindernia, and we can report that it works for all of them. Overall, I am satisfied with this antibody from Agrisera.	2013-08-19 @ 19:45:35
Miroslava
This antibody has been working well It always gave me strong signal above 30 kDa with normally grown Arabidopsis. After high light treatment, there were two additional bands, less intense one above 60 kDa (D1 aggregate) and faint one @ 25kDa (degradation product). I have been using recommended dilution 1:10000. We were satisfied with this antibody.	2012-09-18 @ 09:06:10
Shizue Matsubara
This antibody gave 2 bands - a clear band at 34 kDa and a faint band at 24 kDa - with low light-grown Arabidopsis leaf protein extracts.	2012-04-04 @ 17:32:27
Elzbieta Romanowska
This antibody has been working well on Panisum miliaceum,Panicum maximum, Zea mays and Pisum sativum. We are satisfied.	2009-06-30 @ 16:29:44
Sergio González-Pérez
We have used this antibody for western blot detection of D1 in Arabidopsis cell cultures and spinach reaction centres. Just mention that we got a very well defined and clear signal even at low dilution (1:20000). It allows a precise quantification.	2009-04-21 @ 10:39:36