Anti-Cu/ZnSOD | Cu/Zn superoxide dismutase

Susanne Braun
We have used PC antibody with protein extrac from Hordeum vulgare.We got a special bandLoad per well: 15µg total Protein, 14% SDS-GelMembrane: NC von Roth, HP40.1Blocken: 5% milk in TBST, 2h, RTCU/ZnSOD ( AS10 652 ): 1:1000 in 2% milk, 1 x TBST, over night, 4°CSecondary antibody : Rabbit anti goat IgG AS 09605 1:50000 in 2% milk / 1 x TBST 1h RT Developing reagent: ECL ( GE Healthcare )1:1:2Exposure time: 1sec.	2017-01-24 @ 13:27:20
Alicja Buczynska
I used this antybody for Cu/ZnSOD immunodetection analysis in the leaves of three grasses: Echinochloa crus-galli, Digitaria sanguinalis and Zea mays plants (all NADP-ME subtype). For each treatment 15 µg of total proteins extract from the leaves were loaded on SDS-PAGE (12% acrylamide) and immunodetected with anti-Cu/ZnSOD (Agrisera). The highest signal was observed in Echinochloa crus-galli, Digitaria sanguinalis, and the weakest signal was noted in Zea mays in different irradiation of the light.	2014-05-08 @ 14:26:49