Cu/ZnSOD | Cu/Zn superoxide dismutase
AS10 652 | Clonality: Polyclonal | Host: Chicken | Reactivity: L. longiflorum, O. europaea, O. sativa, S. lycopersicum, S. oleracea
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15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0
15.3 | 16 kDa (Olea europaea L.)
Ananas ananas, Betula pendula, Camellia sinensis, Codonis lanceolata, Cucurbita ficifolia, Helianthus sp., Hordeum vulgare, Lycopersicum esculentum, Plantago major, Populus trichocarpa, Solanum nigrum, Solanum tuberosum, Solidago sp., Vitis vinifera
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Helianthus annuus , Nicotiana tabacum, Marchantia polymorpha, Musa acuminata
25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad).
The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer.
Courtesy Dr. Juan de Dios Alche, CSIC, Spain
Total IgY concentration is 2.3 mg/ml.
Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.
Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar.
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.
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