Cu/ZnSOD | Cu/Zn superoxide dismutase

AS10 652 | Clonality: Polyclonal | Host: Chicken | Reactivity: L. longiflorum, O. europaea, O. sativa, S. lycopersicum, S. oleracea

Product Information

Immunogen

15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0

Host Chicken

Clonality Polyclonal

Purity Purified, total IgY in 10 mM Tris, 150 mM NaCl, 0.02% sodium azide.

Format Liquid

Quantity 100 µl

Storage Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1500 with Alexa 488 (WB)

Expected | apparent MW

15.3 | 16 kDa (Olea europaea L.)

Reactivity

Confirmed reactivity Arabidopsis thaliana (leaves), Lilium longiflorum, Olea europaea (pollen), Oryza sativa, Spinacia oleracea (leaves), Solanum lycopersicum, Zea mays

Predicted reactivity

Ananas ananas, Betula pendula, Camellia sinensis, Codonis lanceolata, Cucurbita ficifolia, Helianthus sp., Hordeum vulgare, Lycopersicum esculentum, Plantago major, Populus trichocarpa, Solanum nigrum, Solanum tuberosum, Solidago sp., Vitis vinifera

Species of your interest not listed? Contact us

Not reactive in

Helianthus annuus , Nicotiana tabacum, Marchantia polymorpha, Musa acuminata, Vicia faba

Application examples

Application example

western blot using hen anti-Cu Zn SOD antibody

25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad).

The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer.

Courtesy Dr. Juan de Dios Alche, CSIC, Spain

Additional information

Total IgY concentration is 2.3 mg/ml.

Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.

Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar

Background

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Product citations

Selected references Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.

immunogen:	15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0
Host:	Chicken
Clonality:	Polyclonal
Purity:	Purified, total IgY in 10 mM Tris, 150 mM NaCl, 0.02% sodium azide.
Format:	Liquid
Quantity:	100 µl
storage:	Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1500 with Alexa 488 (WB)
calculated \| apparent molecular mass [kDa]:	15.3 \| 16 kDa (Olea europaea L.)
Confirmed reactivity:	Arabidopsis thaliana (leaves), Lilium longiflorum, Olea europaea (pollen), Oryza sativa, Spinacia oleracea (leaves), Solanum lycopersicum, Zea mays
predicted reactivity:	Ananas ananas, Betula pendula, Camellia sinensis, Codonis lanceolata, Cucurbita ficifolia, Helianthus sp., Hordeum vulgare, Lycopersicum esculentum, Plantago major, Populus trichocarpa, Solanum nigrum, Solanum tuberosum, Solidago sp., Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	Helianthus annuus , Nicotiana tabacum, Marchantia polymorpha, Musa acuminata, Vicia faba
Picture (footer):	Application example 25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad). The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer. Courtesy Dr. Juan de Dios Alche, CSIC, Spain
additional information:	Total IgY concentration is 2.3 mg/ml. Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.
additional information (application):	Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar
background:	Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
All references:	Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162. Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478. Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.