Cu/ZnSOD | Cu/Zn superoxide dismutase

Product no: AS10 652

AS10 652  |  Clonality: Polyclonal |  Host: Chicken  |  Reactivity: L. longiflorum, O. europaea, O. sativa, S. lycopersicum, S. oleracea

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  • Product Info
  • Immunogen:

    15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0

    Host: Chicken
    Clonality: Polyclonal
    Purity: Purified, total IgY in 10 mM Tris, 150 mM NaCl, 0.02% sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1500 with Alexa 488 (WB)
    Expected | apparent MW:

    15.3 | 16 kDa (Olea europaea L.)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana (leaves), Lilium longiflorum, Olea europaea (pollen), Oryza sativa, Spinacia oleracea (leaves), Solanum lycopersicum, Zea mays
    Predicted reactivity:

    Ananas ananas, Betula pendula, Camellia sinensis, Codonis lanceolata, Cucurbita ficifolia, Helianthus sp., Hordeum vulgare, Lycopersicum esculentum, Plantago major, Populus trichocarpa, Solanum nigrum, Solanum tuberosum, Solidago sp., Vitis vinifera


    Species of your interest not listed? Contact us
    Not reactive in:

    Helianthus annuus , Nicotiana tabacum, Marchantia polymorpha, Musa acuminata, Vicia faba

  • Application Examples
  • Application example

    western blot using hen anti-Cu Zn SOD antibody

    25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad).


    The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer.

    Courtesy Dr. Juan de Dios Alche, CSIC, Spain

  • Additional Information
  • Additional information:

    Total IgY concentration is 2.3 mg/ml.

    Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.

    Additional information (application): Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar
  • Background
  • Background:

    Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

  • Product Citations
  • Selected references: Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.
    Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
    Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
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    Number of reviews: (2)
    Susanne Braun | 2017-01-24
    0 | 0
    We have used PC antibody with protein extrac from Hordeum vulgare.We got a special bandLoad per well: 15g total Protein, 14% SDS-GelMembrane: NC von Roth, HP40.1Blocken: 5% milk in TBST, 2h, RTCU/ZnSOD ( AS10 652 ): 1:1000 in 2% milk, 1 x TBST, over night, 4CSecondary antibody : Rabbit anti goat IgG AS 09605 1:50000 in 2% milk / 1 x TBST 1h RT Developing reagent: ECL ( GE Healthcare )1:1:2Exposure time: 1sec.
    Alicja Buczynska | 2014-05-08
    0 | 0
    I used this antybody for Cu/ZnSOD immunodetection analysis in the leaves of three grasses: Echinochloa crus-galli, Digitaria sanguinalis and Zea mays plants (all NADP-ME subtype). For each treatment 15 g of total proteins extract from the leaves were loaded on SDS-PAGE (12% acrylamide) and immunodetected with anti-Cu/ZnSOD (Agrisera). The highest signal was observed in Echinochloa crus-galli, Digitaria sanguinalis, and the weakest signal was noted in Zea mays in different irradiation of the light.

Accessories

AS06 125  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. maritima, A. thaliana, B. juncea, Ch. reinhardtii, D. bardawil, D.salina, M. sativa, Morus spp.,O. sativa, Salicornia sp., S. tuberosum, winter triticale, Z. mays

302 €
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS06 170 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.maritima, B.juncea, D.sanguinalis, E.crus-galli, E.tef, I.pumila, I. walleriana, L.formosana, N.cataria, M. sativa, N.rtanjensis, O.sativa, P.Sogo Yukidian cultivar V3, P.vulgaris, P.sativum, Populus sp., S.superba, S.lycopersicum, S.tuberosum, Z.mays

302 €
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS09 603 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Hen IgY (H&L)

10 % until end of 2023. Use discount code: Conj10

206 €
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