CSD2 | Chloroplastic Cu/Zn superoxide dismutase

AS06 170 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.maritima, B.juncea, D.sanguinalis, E.crus-galli, E.tef, I.pumila, I. walleriana, L.formosana, N.cataria, M. sativa, N.rtanjensis, O.sativa, P.Sogo Yukidian cultivar V3, P.vulgaris, P.sativum, Populus sp., S.superba, S.lycopersicum, S.tuberosum, Z.mays

CSD2 | Chloroplastic Cu/Zn superoxide dismutase

Product Information

Immunogen

overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000-1 : 4000 (WB)

Expected | apparent MW

22 | 19 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Armeria maritima, Brassica juncea, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Impatiens walleriana, Iris pumila, Liquidambar formosana, Medicago sativa,Musa acuminate, Musa paradisiaca L., Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays

Predicted reactivity Cucumis melo, Glycne max, Pinus pinaster, Spinacia oleracea

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Hordeum vulgare (Barley)

Application: Western Blotting

Pudmed ID: 28338757

Journal: J Exp Bot

Figure Number: 6A

Published Date: 2017-02-01

First Author: Kucharewicz, W., Distelfeld, A., et al.

Impact Factor: 6.088

Open Publication

Immunological analyses of the relative amounts of photosynthesis-associated proteins during senescence of primary leaves from wild-type (WT) and RNAi-W1-7 plants grown at high irradiance. Samples were prepared from wild-type plants grown for 10, 14, 21, 26, 29, and 31 das, as well as from RNAi-W1-7 plants grown for 10, 14, 21, 24, 29, 31, 34, and 36 das. The analysis was done with two biological replicates. WHIRLY1, photosynthesis-related proteins, and Cu/ZnSOD were detected by specific antibodies.

Additional information

This product can be sold containing ProClin if requested.

Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form

Background

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Product citations

Selected references Zhuang et al (2021). EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis. Cell Rep. 2021 Jul 13;36(2):109384. doi: 10.1016/j.celrep.2021.109384. PMID: 34260941.
Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.
Fesharaki-Esfahani et al. (2021) A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina,Phytochemistry,Volume 187,2021,112766,ISSN 0031-9422,https://doi.org/10.1016/j.phytochem.2021.112766.
Konkolewska et al. (2020). Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd).
Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.
Mermod et al. (2019). SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019 May 15. doi: 10.1007/s00299-019-02422-0.
Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.
Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19
Dmitrovi? et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64
Tuteja et al. (2015). Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum. BMC Biotechnology 2015, 15:3 doi:10.1186/s12896-015-0117-0
Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.
Mom?ilovi? et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.
Yang et al. (2012). Quantitative proteomic analysis reveals that antioxidation mechanisms contribute to cold tolerance in plantain (Musa paradisiaca L.; ABB Group) seedlings. Mol Cell Proteomics. 2012 Dec;11(12):1853-69. doi: 10.1074/mcp.M112.022079.

More images:	Reactant: Hordeum vulgare (Barley) Application: Western Blotting Pudmed ID: 28338757 Journal: J Exp Bot Figure Number: 6A Published Date: 2017-02-01 First Author: Kucharewicz, W., Distelfeld, A., et al. Impact Factor: 6.088 Open Publication Immunological analyses of the relative amounts of photosynthesis-associated proteins during senescence of primary leaves from wild-type (WT) and RNAi-W1-7 plants grown at high irradiance. Samples were prepared from wild-type plants grown for 10, 14, 21, 26, 29, and 31 das, as well as from RNAi-W1-7 plants grown for 10, 14, 21, 24, 29, 31, 34, and 36 das. The analysis was done with two biological replicates. WHIRLY1, photosynthesis-related proteins, and Cu/ZnSOD were detected by specific antibodies.
Confirmed reactivity:	Arabidopsis thaliana, Armeria maritima, Brassica juncea, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Impatiens walleriana, Iris pumila, Liquidambar formosana, Medicago sativa,Musa acuminate, Musa paradisiaca L., Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays
predicted reactivity:	Cucumis melo, Glycne max, Pinus pinaster, Spinacia oleracea Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	This product can be sold containing ProClin if requested.
additional information (application):	Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form
background:	Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
All references:	Zhuang et al (2021). EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis. Cell Rep. 2021 Jul 13;36(2):109384. doi: 10.1016/j.celrep.2021.109384. PMID: 34260941. Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507. Fesharaki-Esfahani et al. (2021) A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina,Phytochemistry,Volume 187,2021,112766,ISSN 0031-9422,https://doi.org/10.1016/j.phytochem.2021.112766. Konkolewska et al. (2020). Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd). Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522. Mermod et al. (2019). SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019 May 15. doi: 10.1007/s00299-019-02422-0. Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008. Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19 Dmitrovi? et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64 Tuteja et al. (2015). Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum. BMC Biotechnology 2015, 15:3 doi:10.1186/s12896-015-0117-0 Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29. Mom?ilovi? et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066. Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014. Yang et al. (2012). Quantitative proteomic analysis reveals that antioxidation mechanisms contribute to cold tolerance in plantain (Musa paradisiaca L.; ABB Group) seedlings. Mol Cell Proteomics. 2012 Dec;11(12):1853-69. doi: 10.1074/mcp.M112.022079.
calculated \| apparent molecular mass [kDa]:	22 \| 19 kDa (Arabidopsis thaliana)
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000-1 : 4000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)