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FeSOD | Chloroplastic Fe-dependent superoxide dismutase

AS06 125  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. maritima, A. thaliana, Ch. reinhardtii, D. bardawil, D.salina, Morus spp.,O. sativa, Salicornia sp., S. tuberosum, winter triticale, Z. mays

FeSOD | Chloroplastic Fe-dependent superoxide dismutase in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS06 125)

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Product Information

Immunogen

overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1500-1 : 5000 (WB)
Expected | apparent MW

25 | 22 kDa

Reactivity

Confirmed reactivity Armeria maritima, Arabidopsis thaliana, Chlamydomonas reinhardtii, Dunaliella bardawil, Dunaliella salina, Morus spp., Oryza sativa, Salicornia sp., Solanum tuberosum, winter triticale, Zea mays
Predicted reactivity

Algae, Dunaliella salina, Glycine max, Solanum lycopersicum,  Physcomitrella patens, Pinus pinaster, Populus balsamifera, Volvox carteri

Not reactive in Cyanobacteria

Application examples

Application examples

Application example

Western blot using anti-FeSOD antibodies

5 µg of stromal protein from (1) Chlamydomonas reinhardtii (left), (2) Arabidopsis thaliana were separated on  SDS-PAGE. Primary antibodies have been used in 1: 3000.  

Additional information

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

Out of three FeSOD isoforms, FeSOD2 and FeSOD3 are not expressed in the roots. In roots of Arabidopsis thaliana, FeSOD1 is detected Takáč et al. (2018)

This product can be sold containing ProClin if requested

Related products

Related products

AS06 170 | Anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase, rabbit antibodies

AS07 219 | Anti-CCS | chloroplastic copper chaperone for SOD, rabbit antibodies

AS09 524 | Anti-MnSOD | manganese superoxide dismutase, rabbit antibodies

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer 

Background

Background

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Product citations

Selected references Chen et al. (2018). The molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition. Sci Rep. 2018 Aug 14;8(1):12120. doi: 10.1038/s41598-018-30379-9. Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Hura et al. (2018). Rieske iron-sulfur protein of cytochrome-b6f is involved in plant recovery after drought stress. Rieske iron-sulfur protein of cytochrome-b6f is involved in plant recovery after drought stress.
Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.
Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.
Volgusheva et al. (2017). Comparative analyses of H2 photoproduction in magnesium- and sulfur-starved Chlamydomonas reinhardtii cultures. Physiol Plant. 2017 Apr 7. doi: 10.1111/ppl.12576.
Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.

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