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MnSOD | Manganese superoxide dismutase

AS09 524 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. maritima, B.napus, B. oleracea, N. cataria, N. rtanjensis, O. sativa, P. sativum, Salicornia sp., S. tuberosum

MnSOD | Manganese superoxide dismutase in the group Antibodies for Plant/Algal  / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS09 524)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from available MnSOD sequences in di and monocotyl plants including Arabidopsis thaliana O81235, At3g10920

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000-1 : 5000 (WB)
Expected | apparent MW

25 | 25 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Armeria maritima, Brassica napus, Brassica oleracea, Iris pumila, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Pisum sativum, Salicornia sp. , Solanum tuberosum
Predicted reactivity Gossipium mexicanum, Hordeum vulgare, Musa acuminata, Picea sitcHensis, Populus balsamifera sub. trichocarpa, Raphanus sativus, Solanum tuberosum, Triticum aestivum, Vitis vinifera, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example


western blot using anti plant MnSOD antibody

5 µg (1,2), 10 µg (3, 4) of total protein from Pisum sativum were separated on 12% SDS-PAGE and blotted 30 min. to PVDF. Blots were blocked (in 5% fat free milk) immediately following transfer in for 1h at RT with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 5 min in TBS-T at RT with agitation. Blots were incubated in secondary antibody (anti- IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 for 1h at RT with agitation. The blots were washed 4 times for 5 min in TBS-T and 2 times for 5 min in TBS and developed for 1 min with ECL detection reagent according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesy Dr. Elżbieta Romanowska, Warsaw University, Poland

Additional information

Additional information

Freshly prepared reducing agent like DTT needs to be used in a sample buffer. Otherwise MnSOD will migrate at 50 kDa.

Related products

Related products

AS06 125 | Anti-FeSOD | chloroplastic Fe-dependent superoxide dismutase, rabbit antibodies

AS06 170 | Anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase, rabbit antibodies

AS07 219 | Anti-CCS | chloroplastic copper chaperone for SOD, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

Plant MnSOD catalyzes the dismutation of superoxide to molecular oxygen and water. One subunit of the homotetramer complex binds 1 Mn cofactor. Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD. Synonymes: MSD1, SODA, SOD3

Product citations

Selected references Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013
Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe DBalážová rought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).
Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19
Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.
Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.
Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.
Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.

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