Anti-ACT | Actin (polyclonal)
329 €
Product no: AS13 2640
| Peter Ma |  |
| This antibody works very well for our samples. The total proteins from the Arabidopsis roots were subjected to 10% SDS-PAGE and then detected with this antibody (1:2500). The result shows that this antibody is quite specific and sensitive. | 2023-08-22 @ 06:59:32 |
| Ayten Ulusan | |
| Very good | 2019-12-16 @ 16:14:47 |
| Micha? Tracz | |
| Used in dillution of 1:5000 in 0.5 % Milk TBS. Short labeling time is possibile (~ 1 - 1.5 hours). I was using a load equiavelnt of total protein extracted from 1.5 mg leaf wet weight per lane. Some backround bands were detectable, however I believe over 90 % of the signal came from the actin monomer. | 2019-11-26 @ 13:26:27 |
| Martiniano Ricardi | |
| We used this antibody diluted 1:2500 in TBS Tween20 against A. thaliana leave extract to detect purity of fractioning experiments.This AB works quite nice. | 2017-12-06 @ 12:14:29 |
| Francesca | |
| We succesfully used this ab diluted 1:2500 against S. lycopersicon leaves extracts. Probing was conducted for 1 h in PBS 5% skim milk. | 2014-11-09 @ 21:07:41 |
| D.sa Valeria Banti | |
| The antibody ACT AS13 2640, made to a recombinant actin, gives a good signal and no nonspecific bands are visible after 100sec of exposition with Extra ensitive ECL (LiteABlot Extra Sensitive Substrate, Euroclone).The immunoblot signal is clearly visible loading 20microgG (maybe less is still ok) of total protein extracted from 4-days-old Arabidopsis thaliana seedling tissue, using 1:5.000 diluition for the primary antibody, the same for the secondary one.In a gradient of protein concentration loaded on 10% SDS polyacrylamide gel (20, 40, 80 microG), the signal increases in a proprortional way and the signal for actin is clear at the expected molecular weight (between 42-45KDa). | 2014-04-03 @ 10:19:40 |
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