Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

Saskia Zeilfelder
We analyzed whole cell samples of Chlamydomonas reinhardtii using 10 µg total protein or 1 µg total chlorophyll per lane. After WB transfer we used an AB dilution of 1:10.000. The product worked really well and we obtained signals at approximately 32 kDa.	2024-07-19 @ 13:52:05
Melanue Internizzi
We used this antibody on chlamydomanas protein extract. With a 1:1000 dilution we get a verry strong signal. I recommand it.	2023-07-26 @ 16:59:56
Benjamin Spaniol
We used this antibody for Chlamydomonas reinhardtii whole cell protein extracts. After separating ~5 µg proteins via SDS-PAGE and western blotting, a dilution of 1:10.000 of the antibody resulted in a clear signal without any additional bands. Definitely recommended!	2021-02-18 @ 12:45:54
Brendan
Antibody works great with both Arabidopsis and Chlamydomonas total protein samples using a 1:10,000 dilution. Results are consistently without high background noise or extra bands.	2015-07-15 @ 21:54:49
Dimitris Petroutsos
4 µg of total protein from Phaeodactylum tricornutum were loaded on a 13% SDS-PAGE and blotted 1.5 h to nitrocellulose. Blots were blocked immediately following transfer in 5% milk-TBS-T for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1:5000 overnight at 4 oC with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed 60 sec with ECL detection reagent. Images of the blots were obtained using a CCD imager (Chemidock MP Imaging, Bio-Rad).	2014-12-20 @ 22:59:57