Name:
Phone:
E-mail:
Address:


 


Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

AS06 119 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, C. quitensis Kunt Bartl, C. pumilum,  H. vulgare, N. gaditana, N. tabacum, P. patens, S. martensi, Synechocystis 6803 substrain PCC-M, U. prolifera

Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal) in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS06 119)

DATA SHEET IN PDF

Qty: 
381
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

GST fusion to cytochrome f from Chlamydomonas reinhartii P23577

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000-1 : 50 000 (WB)
Expected | apparent MW

34 | 31-32 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, CRaterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Nostoc sp. PCC7120, Physcomitrella patens, Selaginella martensi, Synechocystis sp. 6803 substrain PCC-M, Ulva prolifera
Predicted reactivity Algae, Citrus x limon, Zea mays
Species of your interest not listed? Contact us

Application examples

Application examples

Application example

 

Western blot detection using anti-cytochrome f antibodies

Chlamydomonas reinhardtii samples are lysed cells, Arabidopsis thaliana samples are prepared from thylakoid membranes; Samples were boiled 1 min in 3%SDS,then 10ug of chlorophylls were loaded in each well of a 12% acrylamide gel.


Western blot using anti-cyt f antibodies

15 µg protein of whole cell extracts precipitated with 80 % acetone followed by re-suspension in 100 mM Tris HCl pH 6.8, 4 % SDS, 20 mM EDTA of Chlamydomonas reinhardtii were loaded on BioRad 4-20% Criterion TGX Precast Mini protein gels. Gels were run at 85V. For electro-blotting wet transfer onto nitrocellulose membrane was performed (105 V for 1h). Overnight blocking with 5% milk in TBS-T in the cold room. Two-hour-long incubation with anti-cyt f antibodies at a dilution of 1:50 000 in TBS-T was followed by washes and incubation with matching anti-rabbit secondary antibodies and development with chemiluminescent detection reagent following manufacture's recommendations. Image was taken using Chemidoc MP Imaging system. Exposure time was in a range of 3 to 10 seconds. 

Courtesy of Dr. Angeliki Tsichla, Petroutsos lab, CEA Grenoble, France

Additional information

Additional information Contains 0.02 % sodium azide as preservative.
Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.

Related products

Background

Background

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Product citations

Related products: Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS08 306 | Clonality: Polyclonal | Host: Rabbit | reactiviy: A. thaliana, E. crus-galli, H. vulgar...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Off...
194 €