Anti-COXII | Plant Cytochrome oxidase subunit II (affinity purified)

Jane Lee
This antibody works very well for our samples.	2023-12-07 @ 03:42:40
G.Vanlerberghe
This antibody worked effectively in Nicotiana tabacum, using isolated leaf mitochondria.	2018-02-21 @ 16:21:59
Michal RUREK
10 µg of mitochondrial proteins from cauliflower (Brassica oleracea var. botrytis) curds (apical layer) was separated on 12 % SDS-PAGE (Laemmli- type) and blotted 1h to Immobilone P (Millipore) using Sedryt apparatus (Kucharczyk). After blocking (5% milk in PBS-T) for 1 h at RT, blots were incubated in the primary antibody at a dilution of 1: 1000 for overnight at 4 deg. with agitation. The primary antibody, diluted in 2% milk in PBS-T was reused several times. Secondary, anti-rabbit HRP- linked antibodies were bound at 1/10000 dilution at RT (1h). Single band of ca. 30 kDa was detected using standart GE Healthcare ECL reagents.	2014-02-14 @ 16:09:55
Gerhard Obermeyer
The antibody worked well in Western blots and gave a main signal at ca. 30 kDa in a microsomal fraction prepared from Lilium pollen grains. Anti-COXII was diluted 1:1,000 and an anti-chicken IgY HRP-conjugated (1:10,000) was used for detection.	2009-11-18 @ 14:29:21
David Macherel
The antibody yields a clean signal on western blots with extracts from germinating Arabidopsis seeds, young seedlings and leaves. We use it at 1/1000 dilution followed by ECL detection	2009-07-23 @ 15:07:08