Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5

Jane Lee
This antibody works very well for our samples so we are very satisfied with this	2023-12-07 @ 03:44:01
Peter Ma
This antibody works very well for our samples. The membrane proteins from the Arabidopsis roots are subjected to 10% SDS-PAGE and then detected with this antibody (1:5000). The results shows that this antibody is quite specific and sensitive.	2019-01-09 @ 09:17:18
Michal RUREK
15 µg of mitochondrial proteins from cauliflower (Brassica oleracea var. botrytis) curds (apical layer) was separated on 12 % SDS-PAGE (Laemmli- type) and blotted 1h to Immobilone P (Millipore) using Sedryt apparatus (Kucharczyk). After blocking (5% milk in PBS-T) for 1 h at RT, blots were incubated in the primary antibody at a dilution of 1/1000 (could be shifted down to 1/10000) for overnight at 4 deg. with agitation. The primary antibody, diluted in 2% milk in PBS-T was reused several times. Secondary, anti-rabbit HRP- linked antibodies were bound at 1/10000 dilution at RT (1h). Single band of ca. 30 kDa was detected using standart GE Healthcare ECL reagents.	2014-02-14 @ 16:13:19
David Macherel
The antibody yields good signal on western blots with extracts from germinating Arabidopsis seeds and young seedlings. We use it at 1/1000 dilution followed by ECL detection	2009-07-23 @ 15:05:01
Emanuel Schmid
We did first attempts in our lab with the previous version of this antibody. It worked fine for enriched Mitochondria samples but unfortunately not in crude Arabidopsis samples. This new version of the VDAC1 antibody solves completely the previous problems, We were now able to detect the VDAC1 even in crude Arabidopsis samples.-------------------------------------------Materials and Methods:Crude Arabidopsis extract was obtained by blending leaves and filtering it through Miracloth. 7 microgramm protein of this mix was used for the SDS-Page.Blocking overnight @ 4 °C in 5% milk in PBS. Antibody incubation for 3h @ RT, 1:1000 in PBS 5% milk. Detection with Protein A.	2009-03-05 @ 16:03:37
Bronwyn Barkla
We are very satisfied with this antibody.	2008-11-17 @ 19:47:32