Anti-ACO1 | Aconitase

Product no: AS09 521
Michal RUREK
10 µg of mitochondrial proteins from cauliflower (Brassica oleracea var. botrytis) curds (apical layer) was separated on 12 % SDS-PAGE (Laemmli- type) and blotted 1h to Immobilone P (Millipore) using Sedryt apparatus (Kucharczyk). After blocking (5% milk in PBS-T) for 1 h at RT, blots were incubated in the primary antibody at a dilution of 1: 10000 for 1h at room temperature with agitation. The primary antibody, diluted in 2% milk in PBS-T was reused several times. Secondary, anti-rabbit HRP- linked antibodies were bound at 1/10000 dilution. Single band of ca. 100 kDa was detected using standart GE Healthcare ECL reagents. 2014-02-14 @ 14:19:39

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