Anti-HSP70 | Heat shock protein 70 (cytoplasmic)
Alberto Dávalos | ![]() |
This antibody worked very well. Dilution 1:2000. We propose as a marker of plant-derived extracellular vesicles. It works in A. thaliana, broccoli, pomegranate and orange. Pharmacol Res. 2023 Dec198:106999. doi: 10.1016/j.phrs.2023.106999. | 2024-04-12 @ 13:36:35 |
Shelby Hotz | ![]() |
For my lab's study, we used this antibody for western blots to detect the abundance of Hsp70 in heat shocked and non-heat shocked bull kelp (Nereocystis leutkeana). We used a 1:3000 dilution in a 5% Nonfat Dried Milk in 1x Tris Buffered Saline solution. The results were positive, with the protein bands showing up clearly and at the correct weight according to the positive control we used. I am happy to report the antibody has been working effectively and as intended in our molecular research. The instructions that come with the product are very comprehensive and relatively easy to follow, and doing so has allowed us to effectively use the materials as expected and needed. We also appreciate the promptness of the delivery and arrival of the materials, and having the antibody in a lyophilized form is very much appreciated. Thank you for your service. | 2023-03-21 @ 18:57:25 |
?udmila Holubová | ![]() |
We are very satisfied with this antibody. We used it in Zea mays protein extract in dilution 1:3000, but I agree, it could be used also in higher dilution. | 2019-10-04 @ 13:32:22 |
Luis E Hernandez | ![]() |
We tried with success this antibody in Medicago sativa, with 10 ug protein loading, with a 1/5000 dilution of primary antibody and 1/20000 secondary HRP, suitable for ECL detection. Due its high reactivity, a higher dilution of primary antibody could be tried. | 2011-10-04 @ 22:02:43 |
Dr. Rena Gorovits | ![]() |
Excellent antibody distinguishes between cytoplasmic and nuclear fractions in tomato protein extracts. | 2009-04-30 @ 13:42:18 |
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